TY  - JOUR
AU  - Kulaš, Jelena
AU  - Ninkov, Marina
AU  - Tucović, Dina
AU  - Popov-Aleksandrov, Aleksandra
AU  - Ukropina, Mirela
AU  - Cakić-Milošević, Maja
AU  - Mutić, Jelena
AU  - Kataranovski, Milena
AU  - Mikrov, Ivana
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3455
AB  - Objective The aim of this study is to investigate the effects of oral cadmium (Cd) ingestion on the pulmonary immune response. Methods Determination of Cd content in lungs and histopathological evaluation of the tissue was performed in rats following 30-day oral Cd administration (5 and 50 mg/L). Antioxidant enzyme defense (superoxide dismutase and catalase), cell infiltration, and production of tumor necrosis factor (TNF) and interferon (IFN)-γ, as well as the activity of myeloperoxidase (MPO), nitric oxide (NO), and various cytokines [interleukin (IL)-1β, IL-6, IL-10, and IL-17] were investigated. Results Cd caused tissue damage and cell infiltration in the lungs, and this damage was more pronounced at higher doses. Cd deposition resulted in lung inflammation characterized by a dose-dependent IL-1β increase in lung homogenates, increased TNF levels at both doses, and IL-6 stimulation at low doses with inhibition observed at higher doses. Cd exerted differential effects on lung leukocytes isolated by enzyme digestion, and these effects were characterized by a lack of change in the production of reactive oxygen and nitrogen species, an inhibition of IL-1β and TNF, and stimulation of MPO and IFN-γ. The higher capacity of Cd-exposed lung cells to respond to the opportunistic pathogen Staphylococcus epidermidis was demonstrated in vitro. Conclusion The potential of ingested Cd to exert both proinflammatory and immunosuppressive effects on pulmonary tissue inflammation and immune reactivity highlights the complex immunomodulatory actions of this metal.
T2  - Biomedical and environmental sciences : BES
T1  - Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats
VL  - 32
IS  - 7
SP  - 508
EP  - 519
DO  - 10.3967/bes2019.068
ER  - 

@article{
author = "Kulaš, Jelena and Ninkov, Marina and Tucović, Dina and Popov-Aleksandrov, Aleksandra and Ukropina, Mirela and Cakić-Milošević, Maja and Mutić, Jelena and Kataranovski, Milena and Mikrov, Ivana",
year = "2019",
abstract = "Objective The aim of this study is to investigate the effects of oral cadmium (Cd) ingestion on the pulmonary immune response. Methods Determination of Cd content in lungs and histopathological evaluation of the tissue was performed in rats following 30-day oral Cd administration (5 and 50 mg/L). Antioxidant enzyme defense (superoxide dismutase and catalase), cell infiltration, and production of tumor necrosis factor (TNF) and interferon (IFN)-γ, as well as the activity of myeloperoxidase (MPO), nitric oxide (NO), and various cytokines [interleukin (IL)-1β, IL-6, IL-10, and IL-17] were investigated. Results Cd caused tissue damage and cell infiltration in the lungs, and this damage was more pronounced at higher doses. Cd deposition resulted in lung inflammation characterized by a dose-dependent IL-1β increase in lung homogenates, increased TNF levels at both doses, and IL-6 stimulation at low doses with inhibition observed at higher doses. Cd exerted differential effects on lung leukocytes isolated by enzyme digestion, and these effects were characterized by a lack of change in the production of reactive oxygen and nitrogen species, an inhibition of IL-1β and TNF, and stimulation of MPO and IFN-γ. The higher capacity of Cd-exposed lung cells to respond to the opportunistic pathogen Staphylococcus epidermidis was demonstrated in vitro. Conclusion The potential of ingested Cd to exert both proinflammatory and immunosuppressive effects on pulmonary tissue inflammation and immune reactivity highlights the complex immunomodulatory actions of this metal.",
journal = "Biomedical and environmental sciences : BES",
title = "Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats",
volume = "32",
number = "7",
pages = "508-519",
doi = "10.3967/bes2019.068"
}

Kulaš, J., Ninkov, M., Tucović, D., Popov-Aleksandrov, A., Ukropina, M., Cakić-Milošević, M., Mutić, J., Kataranovski, M.,& Mikrov, I.. (2019). Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats. in Biomedical and environmental sciences : BES, 32(7), 508-519.
https://doi.org/10.3967/bes2019.068

Kulaš J, Ninkov M, Tucović D, Popov-Aleksandrov A, Ukropina M, Cakić-Milošević M, Mutić J, Kataranovski M, Mikrov I. Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats. in Biomedical and environmental sciences : BES. 2019;32(7):508-519.
doi:10.3967/bes2019.068 .

Kulaš, Jelena, Ninkov, Marina, Tucović, Dina, Popov-Aleksandrov, Aleksandra, Ukropina, Mirela, Cakić-Milošević, Maja, Mutić, Jelena, Kataranovski, Milena, Mikrov, Ivana, "Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats" in Biomedical and environmental sciences : BES, 32, no. 7 (2019):508-519,
https://doi.org/10.3967/bes2019.068 . .

TY  - JOUR
AU  - Tucović, Dina
AU  - Popov-Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Kulaš, Jelena
AU  - Zolotarevski, Lidija
AU  - Vukojević, Vesna
AU  - Mutić, Jelena
AU  - Tatalović, Nikola
AU  - Kataranovski, Milena
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/339
AB  - Skin can acquire cadmium (Cd) by oral route, but there is paucity of data concerning cutaneous effects of this metal. Cd acquired by oral route can affect skin wound healing, but the effect of Cd on other activities involved in skin homeostasis, including skin immunity, are not explored. Using the rat model of 30–day oral administration of Cd (5 ppm and 50 ppm) in drinking water, basic aspects of immune-relevant activity of epidermal cells were examined. Dose-dependent Cd deposition in the the skin was observed (0.035 ± 0.02 µg/g and 0.127 ± 0.04 µg/g at 5 ppm and 50 ppm, respectively, compared to 0.012 ± 0.009 µg/g at 0 ppm of Cd). This resulted in skin inflammation (oxidative stress at both Cd doses and dose-dependent structural changes in the skin and the presence/activation of innate immunity cells). At low Cd dose inflammatory response (nitric oxide and IL-1β) was observed. Other inflammatory cytokines (IL-6 and TNF) response occurred at 50 ppm, which was increased further following skin sensitization with contact allergen dinitro-chlorobenzene (DNCB). Epidermal cells exposed to both Cd doses enhanced concanavalin A (ConA)-stimulated lymphocyte production of IL-17. This study showed for the first time the effect of the metal which gained access to the skin via gut on immune reactivity of epidermal cells. Presented data might be relevant for the link between dietary Cd and the risk of skin pathologies. © 2018 Elsevier Inc.
T2  - Ecotoxicology and Environmental Safety
T1  - Oral cadmium exposure affects skin immune reactivity in rats
VL  - 164
SP  - 12
EP  - 20
DO  - 10.1016/j.ecoenv.2018.07.117
ER  - 

@article{
author = "Tucović, Dina and Popov-Aleksandrov, Aleksandra and Mirkov, Ivana and Ninkov, Marina and Kulaš, Jelena and Zolotarevski, Lidija and Vukojević, Vesna and Mutić, Jelena and Tatalović, Nikola and Kataranovski, Milena",
year = "2018",
abstract = "Skin can acquire cadmium (Cd) by oral route, but there is paucity of data concerning cutaneous effects of this metal. Cd acquired by oral route can affect skin wound healing, but the effect of Cd on other activities involved in skin homeostasis, including skin immunity, are not explored. Using the rat model of 30–day oral administration of Cd (5 ppm and 50 ppm) in drinking water, basic aspects of immune-relevant activity of epidermal cells were examined. Dose-dependent Cd deposition in the the skin was observed (0.035 ± 0.02 µg/g and 0.127 ± 0.04 µg/g at 5 ppm and 50 ppm, respectively, compared to 0.012 ± 0.009 µg/g at 0 ppm of Cd). This resulted in skin inflammation (oxidative stress at both Cd doses and dose-dependent structural changes in the skin and the presence/activation of innate immunity cells). At low Cd dose inflammatory response (nitric oxide and IL-1β) was observed. Other inflammatory cytokines (IL-6 and TNF) response occurred at 50 ppm, which was increased further following skin sensitization with contact allergen dinitro-chlorobenzene (DNCB). Epidermal cells exposed to both Cd doses enhanced concanavalin A (ConA)-stimulated lymphocyte production of IL-17. This study showed for the first time the effect of the metal which gained access to the skin via gut on immune reactivity of epidermal cells. Presented data might be relevant for the link between dietary Cd and the risk of skin pathologies. © 2018 Elsevier Inc.",
journal = "Ecotoxicology and Environmental Safety",
title = "Oral cadmium exposure affects skin immune reactivity in rats",
volume = "164",
pages = "12-20",
doi = "10.1016/j.ecoenv.2018.07.117"
}

Tucović, D., Popov-Aleksandrov, A., Mirkov, I., Ninkov, M., Kulaš, J., Zolotarevski, L., Vukojević, V., Mutić, J., Tatalović, N.,& Kataranovski, M.. (2018). Oral cadmium exposure affects skin immune reactivity in rats. in Ecotoxicology and Environmental Safety, 164, 12-20.
https://doi.org/10.1016/j.ecoenv.2018.07.117

Tucović D, Popov-Aleksandrov A, Mirkov I, Ninkov M, Kulaš J, Zolotarevski L, Vukojević V, Mutić J, Tatalović N, Kataranovski M. Oral cadmium exposure affects skin immune reactivity in rats. in Ecotoxicology and Environmental Safety. 2018;164:12-20.
doi:10.1016/j.ecoenv.2018.07.117 .

Tucović, Dina, Popov-Aleksandrov, Aleksandra, Mirkov, Ivana, Ninkov, Marina, Kulaš, Jelena, Zolotarevski, Lidija, Vukojević, Vesna, Mutić, Jelena, Tatalović, Nikola, Kataranovski, Milena, "Oral cadmium exposure affects skin immune reactivity in rats" in Ecotoxicology and Environmental Safety, 164 (2018):12-20,
https://doi.org/10.1016/j.ecoenv.2018.07.117 . .