TY  - JOUR
AU  - Marić, Nenad
AU  - Matić, Ivan
AU  - Papic, Petar
AU  - Beškoski, Vladimir
AU  - Ilić, Mila V.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Miletić, Srđan B.
AU  - Nikić, Zoran
AU  - Vrvić, Miroslav M.
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2079
AB  - The role of natural attenuation processes in groundwater contamination by petroleum hydrocarbons is of intense scientific and practical interest. This study provides insight into the biodegradation effects in groundwater at a site contaminated by kerosene (jet fuel) in 1993 (Vitanovac, Serbia). Total petroleum hydrocarbons (TPH), hydrochemical indicators (O-2 , NO3-, Mn, Fe, SO42-, HCO3- ), delta C-13 of dissolved inorganic carbon (DIC), and other parameters were measured to demonstrate biodegradation effects in groundwater at the contaminated site. Due to different biodegradation mechanisms, the zone of the lowest concentrations of electron acceptors and the zone of the highest concentrations of metabolic products of biodegradation overlap. Based on the analysis of redox-sensitive compounds in groundwater samples, redox processes ranged from strictly anoxic (methanogenesis) to oxic (oxygen reduction) within a short distance. The dependence of groundwater redox conditions on the distance from the source of contamination was observed. delta C-13 values of DIC ranged from -15.83 to -2.75 parts per thousand, and the most positive values correspond to the zone under anaerobic and methanogenic conditions. Overall, results obtained provide clear evidence on the effects of natural attenuation processes-the activity of biodegradation mechanisms in field conditions.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Natural attenuation of petroleum hydrocarbons-a study of biodegradation effects in groundwater (Vitanovac, Serbia)
VL  - 190
IS  - 2
DO  - 10.1007/s10661-018-6462-4
ER  - 

@article{
author = "Marić, Nenad and Matić, Ivan and Papic, Petar and Beškoski, Vladimir and Ilić, Mila V. and Gojgić-Cvijović, Gordana D. and Miletić, Srđan B. and Nikić, Zoran and Vrvić, Miroslav M.",
year = "2018",
abstract = "The role of natural attenuation processes in groundwater contamination by petroleum hydrocarbons is of intense scientific and practical interest. This study provides insight into the biodegradation effects in groundwater at a site contaminated by kerosene (jet fuel) in 1993 (Vitanovac, Serbia). Total petroleum hydrocarbons (TPH), hydrochemical indicators (O-2 , NO3-, Mn, Fe, SO42-, HCO3- ), delta C-13 of dissolved inorganic carbon (DIC), and other parameters were measured to demonstrate biodegradation effects in groundwater at the contaminated site. Due to different biodegradation mechanisms, the zone of the lowest concentrations of electron acceptors and the zone of the highest concentrations of metabolic products of biodegradation overlap. Based on the analysis of redox-sensitive compounds in groundwater samples, redox processes ranged from strictly anoxic (methanogenesis) to oxic (oxygen reduction) within a short distance. The dependence of groundwater redox conditions on the distance from the source of contamination was observed. delta C-13 values of DIC ranged from -15.83 to -2.75 parts per thousand, and the most positive values correspond to the zone under anaerobic and methanogenic conditions. Overall, results obtained provide clear evidence on the effects of natural attenuation processes-the activity of biodegradation mechanisms in field conditions.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Natural attenuation of petroleum hydrocarbons-a study of biodegradation effects in groundwater (Vitanovac, Serbia)",
volume = "190",
number = "2",
doi = "10.1007/s10661-018-6462-4"
}

Marić, N., Matić, I., Papic, P., Beškoski, V., Ilić, M. V., Gojgić-Cvijović, G. D., Miletić, S. B., Nikić, Z.,& Vrvić, M. M.. (2018). Natural attenuation of petroleum hydrocarbons-a study of biodegradation effects in groundwater (Vitanovac, Serbia). in Environmental Monitoring and Assessment
Springer, Dordrecht., 190(2).
https://doi.org/10.1007/s10661-018-6462-4

Marić N, Matić I, Papic P, Beškoski V, Ilić MV, Gojgić-Cvijović GD, Miletić SB, Nikić Z, Vrvić MM. Natural attenuation of petroleum hydrocarbons-a study of biodegradation effects in groundwater (Vitanovac, Serbia). in Environmental Monitoring and Assessment. 2018;190(2).
doi:10.1007/s10661-018-6462-4 .

Marić, Nenad, Matić, Ivan, Papic, Petar, Beškoski, Vladimir, Ilić, Mila V., Gojgić-Cvijović, Gordana D., Miletić, Srđan B., Nikić, Zoran, Vrvić, Miroslav M., "Natural attenuation of petroleum hydrocarbons-a study of biodegradation effects in groundwater (Vitanovac, Serbia)" in Environmental Monitoring and Assessment, 190, no. 2 (2018),
https://doi.org/10.1007/s10661-018-6462-4 . .

TY  - JOUR
AU  - Beškoski, Vladimir
AU  - Miletić, Srđan B.
AU  - Ilić, Mila V.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Papić, Petar
AU  - Marić, Nenad
AU  - Šolević-Knudsen, Tatjana
AU  - Jovančićević, Branimir
AU  - Nakano, Takeshi
AU  - Vrvić, Miroslav M.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2436
AB  - The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.
PB  - Wiley, Hoboken
T2  - CLEAN - Soil Air Water
T1  - Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater
VL  - 45
IS  - 2
DO  - 10.1002/clen.201600023
ER  - 

@article{
author = "Beškoski, Vladimir and Miletić, Srđan B. and Ilić, Mila V. and Gojgić-Cvijović, Gordana D. and Papić, Petar and Marić, Nenad and Šolević-Knudsen, Tatjana and Jovančićević, Branimir and Nakano, Takeshi and Vrvić, Miroslav M.",
year = "2017",
abstract = "The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.",
publisher = "Wiley, Hoboken",
journal = "CLEAN - Soil Air Water",
title = "Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater",
volume = "45",
number = "2",
doi = "10.1002/clen.201600023"
}

Beškoski, V., Miletić, S. B., Ilić, M. V., Gojgić-Cvijović, G. D., Papić, P., Marić, N., Šolević-Knudsen, T., Jovančićević, B., Nakano, T.,& Vrvić, M. M.. (2017). Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. in CLEAN - Soil Air Water
Wiley, Hoboken., 45(2).
https://doi.org/10.1002/clen.201600023

Beškoski V, Miletić SB, Ilić MV, Gojgić-Cvijović GD, Papić P, Marić N, Šolević-Knudsen T, Jovančićević B, Nakano T, Vrvić MM. Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. in CLEAN - Soil Air Water. 2017;45(2).
doi:10.1002/clen.201600023 .

Beškoski, Vladimir, Miletić, Srđan B., Ilić, Mila V., Gojgić-Cvijović, Gordana D., Papić, Petar, Marić, Nenad, Šolević-Knudsen, Tatjana, Jovančićević, Branimir, Nakano, Takeshi, Vrvić, Miroslav M., "Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater" in CLEAN - Soil Air Water, 45, no. 2 (2017),
https://doi.org/10.1002/clen.201600023 . .

TY  - JOUR
AU  - Marić, Nenad
AU  - Ilić, Mila V.
AU  - Miletić, Srđan B.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav M.
AU  - Papic, Petar
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1958
AB  - This study provides insight into the enhanced in situ bioremediation applied for remediation of groundwater contaminated by petroleum hydrocarbons. Activities prior to the application of this remediation approach included removal of the source of groundwater contamination-an underground storage tank and contaminated sediments-from the unsaturated zone. The hydraulic feasibility of this remediation approach was proved by hydraulic conductivity characterization of the site. Enhanced in situ bioremediation was performed by a combination of biostimulation and bioaugmentation within the closed bipolar system (one extraction and two injection wells). Biostimulation was conducted by addition of nutrients, and stimulation of oxidation processes by injection of H2O2, into the aquifer. Bioaugmentation/re-inoculation was achieved by injection of a zymogenous consortium of hydrocarbon degrading microorganisms isolated from the contaminated groundwater. The average extraction capacity was 0.5 l/s, with an average injection capacity of 0.25 l/s per well. The efficiency of the remediation approach was measured by changes in the content of total petroleum hydrocarbon (TPH), total chemoorganoheterotrophic (TC) and hydrocarbon degrading (HD) microorganisms. After biostimulation and bioaugmentation, the number of TC and HD microorganisms started to increase. This was followed by a decrease of TPH concentration in the groundwater from an initial 6.8-0.5 mg/l at the end of the bioremediation. The applied remediation approach was highly efficient and very effective in reducing TPH to acceptable levels. Together, these facts provide strong evidence of its potential for remediation of groundwater contaminated by petroleum hydrocarbons.
PB  - Springer, New York
T2  - Environmental Earth Sciences
T1  - Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia
VL  - 74
IS  - 6
SP  - 5211
EP  - 5219
DO  - 10.1007/s12665-015-4531-3
ER  - 

@article{
author = "Marić, Nenad and Ilić, Mila V. and Miletić, Srđan B. and Gojgić-Cvijović, Gordana D. and Beškoski, Vladimir and Vrvić, Miroslav M. and Papic, Petar",
year = "2015",
abstract = "This study provides insight into the enhanced in situ bioremediation applied for remediation of groundwater contaminated by petroleum hydrocarbons. Activities prior to the application of this remediation approach included removal of the source of groundwater contamination-an underground storage tank and contaminated sediments-from the unsaturated zone. The hydraulic feasibility of this remediation approach was proved by hydraulic conductivity characterization of the site. Enhanced in situ bioremediation was performed by a combination of biostimulation and bioaugmentation within the closed bipolar system (one extraction and two injection wells). Biostimulation was conducted by addition of nutrients, and stimulation of oxidation processes by injection of H2O2, into the aquifer. Bioaugmentation/re-inoculation was achieved by injection of a zymogenous consortium of hydrocarbon degrading microorganisms isolated from the contaminated groundwater. The average extraction capacity was 0.5 l/s, with an average injection capacity of 0.25 l/s per well. The efficiency of the remediation approach was measured by changes in the content of total petroleum hydrocarbon (TPH), total chemoorganoheterotrophic (TC) and hydrocarbon degrading (HD) microorganisms. After biostimulation and bioaugmentation, the number of TC and HD microorganisms started to increase. This was followed by a decrease of TPH concentration in the groundwater from an initial 6.8-0.5 mg/l at the end of the bioremediation. The applied remediation approach was highly efficient and very effective in reducing TPH to acceptable levels. Together, these facts provide strong evidence of its potential for remediation of groundwater contaminated by petroleum hydrocarbons.",
publisher = "Springer, New York",
journal = "Environmental Earth Sciences",
title = "Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia",
volume = "74",
number = "6",
pages = "5211-5219",
doi = "10.1007/s12665-015-4531-3"
}

Marić, N., Ilić, M. V., Miletić, S. B., Gojgić-Cvijović, G. D., Beškoski, V., Vrvić, M. M.,& Papic, P.. (2015). Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia. in Environmental Earth Sciences
Springer, New York., 74(6), 5211-5219.
https://doi.org/10.1007/s12665-015-4531-3

Marić N, Ilić MV, Miletić SB, Gojgić-Cvijović GD, Beškoski V, Vrvić MM, Papic P. Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia. in Environmental Earth Sciences. 2015;74(6):5211-5219.
doi:10.1007/s12665-015-4531-3 .

Marić, Nenad, Ilić, Mila V., Miletić, Srđan B., Gojgić-Cvijović, Gordana D., Beškoski, Vladimir, Vrvić, Miroslav M., Papic, Petar, "Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia" in Environmental Earth Sciences, 74, no. 6 (2015):5211-5219,
https://doi.org/10.1007/s12665-015-4531-3 . .