Приказ основних података о документу

dc.creatorNikodinović-Runić, Jasmina
dc.creatorMartin, Leona B.
dc.creatorBabu, Ramesh
dc.creatorBlau, Werner
dc.creatorO'Connor, Kevin E.
dc.date.accessioned2018-11-22T00:15:04Z
dc.date.available2018-11-22T00:15:04Z
dc.date.issued2009
dc.identifier.issn0378-1097
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1008
dc.description.abstractTwo melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival rates gt 30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H(2)O(2)) exhibiting 1.5 times smaller zones of inhibition at 10 mM H(2)O(2) compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins.en
dc.publisherWiley-Blackwell Publishing, Inc, Malden
dc.rightsrestrictedAccess
dc.sourceFEMS Microbiology Letters / Federation of European Microbiological Societies
dc.subjectmelaninen
dc.subjecttyrosinaseen
dc.subjectPseudomonas putida F6en
dc.titleCharacterization of melanin-overproducing transposon mutants of Pseudomonas putida F6en
dc.typearticle
dc.rights.licenseARR
dcterms.abstractНикодиновић-Рунић, Јасмина; Блау, Wернер; О'Цоннор, Кевин Е.; Бабу, Рамесх; Мартин, Леона Б.;
dc.citation.volume298
dc.citation.issue2
dc.citation.spage174
dc.citation.epage183
dc.identifier.wos000269056800006
dc.identifier.doi10.1111/j.1574-6968.2009.01716.x
dc.citation.other298(2): 174-183
dc.citation.rankM23
dc.identifier.pmid19624744
dc.description.otherFree full text: [https://doi.org/10.1111/j.1574-6968.2009.01716.x]
dc.type.versionpublishedVersionen
dc.identifier.rcubKon_2008


Документи

Thumbnail

Овај рад се појављује у следећим колекцијама

Приказ основних података о документу