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Characterization of Trypsin-Like Enzymes from the Midgut of Morimus Funereus (Coleoptera: Cerambycidae) Larvae
Karakterizacija tripsinima-sličnih enzima srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)
dc.creator | Lončar, Nikola L. | |
dc.creator | Božić, Nataša | |
dc.creator | Nenadovic, Vera | |
dc.creator | Ivanovic, Jelisaveta | |
dc.creator | Vujčić, Zoran | |
dc.date.accessioned | 2018-11-22T00:15:33Z | |
dc.date.available | 2018-11-22T00:15:33Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 0354-4664 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/1038 | |
dc.description.abstract | The pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine. | en |
dc.description.abstract | pH vrednost duž srednjeg creva larvi M. funereus se razlikuje, kisela je u regionu prednjeg dela, dok je u srednjem i zadnjem delu bazna. Elastazama i himotripsinima slična aktivnost je najveća u srednjem delu srednjeg creva dok je u prednjem delu detektovana mala vrednost, a u zadnjem delu zanemarljiva. Tripsinima slična aktivnost je detektovana duž celog srednjeg creva, s tim da se više od 90 % aktivnosti detektuje u prednjem delu srednjeg creva. Zastupljenost elastazama i himotripsinima sličnih endopeptidaza je zanemarljivo mala u poređenju sa zastupljenošću tripsinima sličnih enzima. U prednjem delu srednjeg creva nalaze se dve izoforme tripsinima sličnih enzima, sa baznim pI vrednostima, koje su skoro u potpunosti inhibirane benzamidinom. | sr |
dc.publisher | Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Archives of biological sciences | |
dc.subject | Cerambycidae | en |
dc.subject | Morimus funereus | en |
dc.subject | Morimus funereus | en |
dc.subject | trypsin | en |
dc.subject | trypsin | en |
dc.subject | midgut peptidases | en |
dc.subject | midgut peptidases | en |
dc.subject | synthetic substrate | en |
dc.subject | synthetic substrate | en |
dc.subject | zymogram | en |
dc.subject | zymogram | en |
dc.title | Characterization of Trypsin-Like Enzymes from the Midgut of Morimus Funereus (Coleoptera: Cerambycidae) Larvae | en |
dc.title | Karakterizacija tripsinima-sličnih enzima srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae) | sr |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Вујчић, Зоран; Бозиц, Натаса; Ивановиц, Јелисавета; Ненадовиц, Вера; Лончар, Никола; | |
dc.citation.volume | 61 | |
dc.citation.issue | 4 | |
dc.citation.spage | 713 | |
dc.citation.epage | 718 | |
dc.identifier.wos | 000273203500018 | |
dc.identifier.doi | 10.2298/ABS0904713L | |
dc.citation.other | 61(4): 713-718 | |
dc.citation.rank | M23 | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-77949879656 | |
dc.identifier.fulltext | https://cherry.chem.bg.ac.rs/bitstream/id/8244/1036.pdf |