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dc.creatorStojanović, Marijana M.
dc.creatorŽivković, Irena
dc.creatorPetrusic, Vladimir Z.
dc.creatorKosec, Dusko J.
dc.creatorDimitrijevic, Rajna D.
dc.creatorJankov, Ratko M.
dc.creatorDimitrijevic, Ljijana A.
dc.creatorGavrović-Jankulović, Marija
dc.date.accessioned2018-11-22T00:15:51Z
dc.date.available2018-11-22T00:15:51Z
dc.date.issued2010
dc.identifier.issn1567-5769
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1043
dc.description.abstractLectins are widely used in many types of assay but some lectins such as banana lectin (BanLec) are recognised as potent immunostimulators Although BanLec's structure and binding characteristics are now familiar, its immunostimulatory potential has not yet been fully explored The synthesis by recombinant technology of a BanLec isoform (rBanLec) whose binding properties are similar to its natural counterpart has made it possible to overcome the twin problems of natural BanLec's microheterogeneity and low availability This study's aim is to explore the immunostimulatory potential of rBanLec in the murine model Analyses of the responses of Balb/c- and C57 BL/6-originated splenocytes to in vitro rBanLec stimulation were performed to examine the dependency of rBanLec's immunostimulatory potential upon the splenocytes' genetic background It is shown that the responses of Balb/c- and C57 BL/6-originated splenocytes to rBanLec stimulation differ both qualitatively and in intensity. The hallmarks of the induced responses are T lymphocyte proliferation and intensive interferon-gamma secretion Both phenomena are more marked in Balb/c-originated cultures; Balb/c-originated lymphocytes produce interleukin (IL)-4 and IL-10 following rBanLec stimulation Out results demonstrate that any responses to rBanLec stimulation are highly dependent upon genetic background. they suggest that genetic background must be an important consideration in any further investigations using animal models or when exploring rBanLec's potential human applications (C) 2009 Elsevier B V. All rights reserveden
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142020/RS//
dc.rightsrestrictedAccess
dc.sourceInternational Immunopharmacology
dc.subjectInterferon-gammaen
dc.subjectMurine lymphocytesen
dc.subjectProliferationen
dc.subjectRecombinant banana lectinen
dc.titleIn vitro stimulation of Balb/c and C57 BL/6 splenocytes by a recombinantly produced banana lectin isoform results in both a proliferation of T cells and an increased secretion of interferon-gammaen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractДимитријевиц, Рајна Д.; Димитријевиц, Љијана A.; Стојановиц, Маријана М.; Гавровић-Јанкуловић, Марија; Косец, Дуско Ј.; Петрусиц, Владимир З.; Зивковиц, Ирена П.; Јанков, Ратко М.;
dc.citation.volume10
dc.citation.issue1
dc.citation.spage120
dc.citation.epage129
dc.identifier.wos000274361700017
dc.identifier.doi10.1016/j.intimp.2009.10.007
dc.citation.other10(1): 120-129
dc.citation.rankM23
dc.identifier.pmid19874914
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-73449101067
dc.identifier.rcubKon_2043


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