Polarographic Assay Based on Hydrogen Peroxide Scavenging in Determination of Antioxidant Activity of Strong Alcohol Beverages
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Novaković, Miroslav M.
Vukosavljevic, Predrag V.
Sunjevic, Desanka Z.
Article (Published version)
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Total antioxidant (AO) activity of strong alcohol beverages such as wine and plum brandies, whiskeys, herbal and sweet fruit liqueurs have been assessed using a polarographic assay based on hydrogen peroxide scavenging (HPS). Rank of order of total AO activity, expressed as percentage of decrease of anodic oxidation current of hydrogen peroxide, was found analogous with total phenolic content estimated by Folin Ciocalteau (FC) assay and radical scavenging capacity against the stable free radical 1,1-dipheny1-2-picrylhydrazyl (DPPH). Application of the assay for surveying of a quarter century long maturation of plum brandy in oak barrel was demonstrated. In addition, influence of different storage conditions on preservation of AO activity of some herbal liqueurs was surveyed. Wide area of application of this simple, fast, low cost and reliable assay in analysis and quality monitoring of various strong alcohol beverages was confirmed.
Keywords:Alcohol beverages / antioxidant activity / Bitter 54 / hydrogen peroxide / phenolics / polarography
Source:Journal of Agricultural and Food Chemistry, 2010, 58, 14, 8400-8406
- Amer Chemical Soc, Washington