Приказ основних података о документу

dc.creatorRadosavljević, Jelena
dc.creatorDobrijevic, Dragana
dc.creatorJadranin, Milka
dc.creatorBlanusa, Milan
dc.creatorVukmirica, Jelena
dc.creatorĆirković-Veličković, Tanja
dc.date.accessioned2018-11-22T00:16:39Z
dc.date.available2018-11-22T00:16:39Z
dc.date.issued2010
dc.identifier.issn0022-5142
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1095
dc.description.abstractBACKGROUND: The major peanut allergens are Ara h 1, Ara h 2 and Ara h 6. Proteolytic processing has been shown to be required for the maturation process of Ara h 6. The aim of this study was to examine whether Ara h 2 undergoes proteolytic processing and, if so, whether proteolytic processing influences its ability to bind human immunoglobulin E (IgE). RESULTS: Ara h 2 isolated from peanut extract under conditions of protease inhibition revealed a single additional peak for its two known isoforms (Ara h 2.01 and Ara h 2.02), corresponding to a C-terminally truncated form lacking a dipeptide (RY). Ara h 2 isolated in the absence of protease inhibition, however, yielded two additional peaks, identified as C-terminally truncated forms lacking either a dipeptide (RY) or a single tyrosine residue. The IgE-binding capacity of the Ara h 2 truncated forms was not altered. CONCLUSION: Ara h 2 undergoes proteolytic processing by peanut proteases that involves C-terminal removal of a dipeptide. Hence Ara h 2 isolated from peanut extract is a complex mixture of two isoforms expressed by different genes, Ara h 2.01 and Ara h 2.02, as well as truncated forms generated by the proteolytic processing of these isoforms. (C) 2010 Society of Chemical Industryen
dc.publisherJohn Wiley & Sons Ltd, Chichester
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142020/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of the Science of Food and Agriculture
dc.subjectAra h 2en
dc.subjectAra h 6en
dc.subjectproteolysisen
dc.subjectIgE bindingen
dc.subjectfood allergyen
dc.titleInsights into proteolytic processing of the major peanut allergen Ara h 2 by endogenous peanut proteasesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЋирковић-Величковић, Тања; Вукмирица, Јелена; Радосављевић, Јелена; Блануса, Милан; Јадранин, Милка; Добријевиц, Драгана;
dc.citation.volume90
dc.citation.issue10
dc.citation.spage1702
dc.citation.epage1708
dc.identifier.wos000280121600018
dc.identifier.doi10.1002/jsfa.4005
dc.citation.other90(10): 1702-1708
dc.citation.rankM21
dc.identifier.pmid20564442
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-77954404613
dc.identifier.rcubKon_2095


Документи

Thumbnail

Овај рад се појављује у следећим колекцијама

Приказ основних података о документу