Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil
Vrvić, Miroslav M.
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Bioremediation potential of bacteria and fungi isolated from sediment samples from an alluvial formation of the Danube (wastewater channel) of the Pancevo Industrial Zone (Serbia) was investigated earlier. (Ilic M., Antic M., Antic V., Schwarzbauer J., Vrvic M. and Jovancicevic B., Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. Environmental Chemistry Letters (submitted)) In this paper, results concerning bioremediation potential of bacteria and fungi isolated from soil samples will be presented. Total isolated microorganisms were divided into three parts and sown in an appropriate medium. Actidione fungicide was added to one innoculum so that only bacteria propagated in it. Streptomycin antibiotic was added to a second innoculum, wherein fungi propagated. The third portion (with no additives) contained consortium of fungi and bacteria. Paraffinic petroleum, in inorganic medium in phosphate puffer, was a substrate for assessment of... bioremediation potential of the mentioned microorganisms. The experiments of the simulated oil biodegradation lasted 30, 60 and 90 days. Extracts were isolated from the samples with chloroform liquid-liquid extraction. Extracts were fractionated by column chromatography. Alkane fraction was analyzed by gas chromatography-mass spectrometry (GC-MS) techniques.