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dc.creatorVujčić, Miroslava
dc.creatorLazic, Milan
dc.creatorMilenković, Milica R.
dc.creatorSladić, Dušan
dc.creatorRadulović, Siniša S.
dc.creatorFilipovic, Nenad
dc.creatorAnđelković, Katarina K.
dc.date.accessioned2018-11-22T00:18:17Z
dc.date.available2018-11-22T00:18:17Z
dc.date.issued2011
dc.identifier.issn1095-6670
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1168
dc.description.abstractOrganometallic Cd(II) compounds have recently attracted attention for their anticancer activity. The interaction of the dinuclear complex of Cd(II) with the condensation product of 2-acetylpyridine and malonic acid dihydrazide, N',N'(2)-bis[(1E)-1-(2pyridyl) ethylidene] propanedihydrazide (Cd(II)H(2)L), with calf thymus DNA (CT-DNA) was monitored by blue shift in UV-vis spectra of the complex. The binding constant of Cd(II) H2L complex with CT-DNA was determined (K(B) = 1.8 x 10(4) M(-1)) and was indicative of minor groove binding. Agarose gel electrophoretic changes in mobility of supercoiled and circular forms of pBR322 and pUC18 plasmids in the presence of the complex suggest that conformational changes in the plasmids occur upon binding of the Cd(II) H2L complex. The Cd(II) H2L complex induced perturbation of the cell cycle phase distribution and an increase in the percentage of cells in the sub-G1 phase of human cervical cancer HeLa cell line and murine melanoma B16 cell line. Immunoblotting analysis showed the overexpression of Bcl-2 protein with the Cd(II)H(2)L complex. (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25: 175-182, 2011; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.20374en
dc.publisherWiley-Blackwell, Malden
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/145035/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Biochemical and Molecular Toxicology
dc.subjectDNA Bindingen
dc.subjectElectrophoretic Analysisen
dc.subjectCell Cycle Phase Perturbationen
dc.titleA Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazideen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМиленковић, Милица; Сладић, Душан; Лазиц, Милан; Вујциц, Мирослава; Aнђелковић, Катарина; Филиповиц, Ненад; Радуловиц, Синиса;
dc.citation.volume25
dc.citation.issue3
dc.citation.spage175
dc.citation.epage182
dc.identifier.wos000291562100007
dc.identifier.doi10.1002/jbt.20374
dc.citation.other25(3): 175-182
dc.citation.rankM23
dc.identifier.pmid21671309
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-79958856469
dc.identifier.rcubKon_2190


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