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dc.creatorĐokić, Lidija
dc.creatorNarančić, Tanja
dc.creatorNikodinović-Runić, Jasmina
dc.creatorSavic, Miloje
dc.creatorVasiljević, Branka
dc.date.accessioned2018-11-22T00:18:32Z
dc.date.available2018-11-22T00:18:32Z
dc.date.issued2011
dc.identifier.issn0175-7598
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1183
dc.description.abstractFour new Gram-positive, phenol-degrading strains were isolated from the rhizospheres of endemorelict plants Ramonda serbica and Ramonda nathaliae known to exude high amounts of phenolics in the soil. Isolates were designated Bacillus sp. PS1, Bacillus sp. PS11, Streptomyces sp. PS12, and Streptomyces sp. PN1 based on 16S rDNA sequence and biochemical analysis. In addition to their ability to tolerate and utilize high amounts of phenol of either up to 800 or up to 1,400 mg l(-1) without apparent inhibition in growth, all four strains were also able to degrade a broad range of aromatic substrates including benzene, toluene, ethylbenzene, xylenes, styrene, halogenated benzenes, and naphthalene. Isolates were able to grow in pure culture and in defined mixed culture on phenol and on the mixture of BTEX (benzene, toluene, ethylbenzene, and xylenes) compounds as a sole source of carbon and energy. Pure culture of Bacillus sp. PS11 yielded 1.5-fold higher biomass amounts in comparison to mixed culture, under all conditions. Strains successfully degraded phenol in the soil model system (2 g kg(-1)) within 6 days. Activities of phenol hydroxylase, catechol 1,2-dioxygenase, and catechol 2,3-dioxygenase were detected and analyzed from the crude cell extract of the isolates. While all four strains use ortho degradation pathway, enzyme indicative of meta degradation pathway (catechol 2,3-dioxygenase) was also detected in Bacillus sp. PS11 and Streptomyces sp. PN1. Phenol degradation activities were induced 2 h after supplementation by phenol, but not by catechol. Catechol slightly inhibited activity of catechol 2,3-dioxygenase in strains PS11 and PN1.en
dc.publisherSpringer, New York
dc.rightsrestrictedAccess
dc.sourceApplied Microbiology and Biotechnology
dc.subjectBiodegradationen
dc.subjectPhenolen
dc.subjectBTEXen
dc.subjectBacillusen
dc.subjectStreptomycesen
dc.subjectSoil model experimentsen
dc.titleIsolation and characterization of four novel Gram-positive bacteria associated with the rhizosphere of two endemorelict plants capable of degrading a broad range of aromatic substratesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractНаранциц, Тања; Никодиновић-Рунић, Јасмина; Савиц, Милоје; Дјокиц, Лидија; Васиљевиц, Бранка;
dc.citation.volume91
dc.citation.issue4
dc.citation.spage1227
dc.citation.epage1238
dc.identifier.wos000293235300035
dc.identifier.doi10.1007/s00253-011-3426-9
dc.citation.other91(4): 1227-1238
dc.citation.rankM21
dc.identifier.pmid21706169
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-80052622153
dc.identifier.rcubKon_2205


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