Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications
Samo za registrovane korisnike
2011
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild-type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells.
Ključne reči:
Directed protein evolution / Glucose oxidase / Glucose oxidation / Electron transfer / Biofuel cells / BiosensorsIzvor:
Applied Biochemistry and Biotechnology, 2011, 165, 7-8, 1448-1457Izdavač:
- Humana Press Inc, Totowa
Finansiranje / projekti:
- Engineering and Physical Sciences Research Council [EP/C535456/1]
- EPSRC [EP/C535456/1]
- US Office of Naval Research (ONR) [N00014-03-1-0026]
Napomena:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3611
DOI: 10.1007/s12010-011-9366-0
ISSN: 0273-2289
PubMed: 21915588
WoS: 000297222900002
Scopus: 2-s2.0-81955167958
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Yu, Eileen Hao AU - Prodanović, Radivoje AU - Gueven, Gueray AU - Ostafe, Raluca AU - Schwaneberg, Ulrich PY - 2011 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1221 AB - In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild-type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells. PB - Humana Press Inc, Totowa T2 - Applied Biochemistry and Biotechnology T1 - Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications VL - 165 IS - 7-8 SP - 1448 EP - 1457 DO - 10.1007/s12010-011-9366-0 ER -
@article{ author = "Yu, Eileen Hao and Prodanović, Radivoje and Gueven, Gueray and Ostafe, Raluca and Schwaneberg, Ulrich", year = "2011", abstract = "In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild-type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells.", publisher = "Humana Press Inc, Totowa", journal = "Applied Biochemistry and Biotechnology", title = "Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications", volume = "165", number = "7-8", pages = "1448-1457", doi = "10.1007/s12010-011-9366-0" }
Yu, E. H., Prodanović, R., Gueven, G., Ostafe, R.,& Schwaneberg, U.. (2011). Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications. in Applied Biochemistry and Biotechnology Humana Press Inc, Totowa., 165(7-8), 1448-1457. https://doi.org/10.1007/s12010-011-9366-0
Yu EH, Prodanović R, Gueven G, Ostafe R, Schwaneberg U. Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications. in Applied Biochemistry and Biotechnology. 2011;165(7-8):1448-1457. doi:10.1007/s12010-011-9366-0 .
Yu, Eileen Hao, Prodanović, Radivoje, Gueven, Gueray, Ostafe, Raluca, Schwaneberg, Ulrich, "Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications" in Applied Biochemistry and Biotechnology, 165, no. 7-8 (2011):1448-1457, https://doi.org/10.1007/s12010-011-9366-0 . .