Приказ основних података о документу
Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications
dc.creator | Yu, Eileen Hao | |
dc.creator | Prodanović, Radivoje | |
dc.creator | Gueven, Gueray | |
dc.creator | Ostafe, Raluca | |
dc.creator | Schwaneberg, Ulrich | |
dc.date.accessioned | 2018-11-22T00:19:08Z | |
dc.date.available | 2018-11-22T00:19:08Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 0273-2289 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/1221 | |
dc.description.abstract | In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild-type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells. | en |
dc.publisher | Humana Press Inc, Totowa | |
dc.relation | Engineering and Physical Sciences Research Council [EP/C535456/1] | |
dc.relation | EPSRC [EP/C535456/1] | |
dc.relation | US Office of Naval Research (ONR) [N00014-03-1-0026] | |
dc.rights | restrictedAccess | |
dc.source | Applied Biochemistry and Biotechnology | |
dc.subject | Directed protein evolution | en |
dc.subject | Glucose oxidase | en |
dc.subject | Glucose oxidation | en |
dc.subject | Electron transfer | en |
dc.subject | Biofuel cells | en |
dc.subject | Biosensors | en |
dc.title | Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Сцхwанеберг, У.; Yу, Еилеен Хао; Остафе, Р.; Продановић, Радивоје; Гуевен, Гуераy; | |
dc.citation.volume | 165 | |
dc.citation.issue | 7-8 | |
dc.citation.spage | 1448 | |
dc.citation.epage | 1457 | |
dc.identifier.wos | 000297222900002 | |
dc.identifier.doi | 10.1007/s12010-011-9366-0 | |
dc.citation.other | 165(7-8): 1448-1457 | |
dc.citation.rank | M23 | |
dc.identifier.pmid | 21915588 | |
dc.description.other | Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3611] | |
dc.type.version | publishedVersion | en |
dc.identifier.scopus | 2-s2.0-81955167958 |