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dc.creatorKovacevic-Filipovic, Milica
dc.creatorIlic, Vesna
dc.creatorVujčić, Zoran
dc.creatorDojnov, Biljana
dc.creatorStevanov-Pavlović, Marija
dc.creatorMijacevic, Zora
dc.creatorBožić, Tatjana T.
dc.date.accessioned2018-11-22T00:20:37Z
dc.date.available2018-11-22T00:20:37Z
dc.date.issued2012
dc.identifier.issn0165-2427
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1257
dc.description.abstractSerum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content. (C) 2011 Elsevier B.V. All rights reserved.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175061/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46009/RS//
dc.rightsrestrictedAccess
dc.sourceVeterinary Immunology and Immunopathology
dc.subjectSerum amyloid Aen
dc.subjectIsoformsen
dc.subjectSubclinical mastitisen
dc.subjectStaphylococcus aureusen
dc.titleSerum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitisen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМијацевиц, Зора; Илиц, Весна; Бозиц, Татјана; Дојнов, Биљана; Вујчић, Зоран; Ковацевиц-Филиповиц, Милица; Стеванов-Павловиц, Марија;
dc.citation.volume145
dc.citation.issue1-2
dc.citation.spage120
dc.citation.epage128
dc.identifier.wos000301159200013
dc.identifier.doi10.1016/j.vetimm.2011.10.015
dc.citation.other145(1-2): 120-128
dc.citation.rankM21
dc.identifier.pmid22100192
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84856555615
dc.identifier.rcubKon_2280


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