Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure
Само за регистроване кориснике
2012
Аутори
Gligorijević, NevenkaAranđelović, Sandra
Filipović, Lana
Jakovljević, Ksenija
Jankovic, Radmila
Grgurić-Šipka, Sanja
Ivanović, Ivanka
Radulović, Siniša
Tešić, Živoslav Lj.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In our previous study, ruthenium(II)-p-cymene complexes of general formula [(eta(6)-p-cymene)Ru(L)Cl2], L: 3-acetylpyridine (1), 2-amino-5-chloropyridine (2); and [(eta(6)-p-cymene)Ru(HL)Cl], HL: 2,3-pyridinedicarboxylic acid (3), 2,4-pyridinedicarboxylic acid (4), revealed low antiproliferative activity, except complex [(eta(6)-p-cymene)RuCl(picolinic acid)]center dot H2O (5) which exhibited IC50 around 80 mu M. In this study we further investigated in vitro potential of antimetastatic action of ruthenium complexes on HeLa and two endothelial cell lines. Comparison of structure and activity of five complexes indicated heterogenic mode of activity, with regard to the potential of antimetastatic and antiproliferative effect. Replacement of substituted pyridine ligand with picolinic acid (complex 5) around Ru(II) center contributed to complex cytotoxicity and ruthenium DNA binding affinity. Analysis of ruthenium(II) accumulation in DNA and protein fractions of HeLa cells, using ICP-OES r...evealed significantly higher content of complex 5 in DNA fraction in comparison to the other tested compounds. It also altered cell cycle progression, affected expression of DNA repair enzymes ERCC1 and MSH2, and showed enhanced activity in combination with 3-aminobenzamide. Regardless of their effect on cell growth, Ru(II) complexes exerted antimetastatic effect on several tumor cell lines in vitro, achieved mostly by the effect on cell adhesion, migration and angiogenesis, while picolinate ruthenium(II)-arene additionally exerted inhibitory effect on extracellular matrix degradation. (c) 2011 Elsevier Inc. All rights reserved.
Кључне речи:
Ruthenium(II)-arene / Antiproliferative activity / Antimetastatic / ERCC1 / PARPИзвор:
Journal of Inorganic Biochemistry, 2012, 108, 53-61Издавач:
- Elsevier Science Inc, New York
Финансирање / пројекти:
- Фармакодинамска и фармакогеномска испитивања новијих лекова у лечењу солидних тумора (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41026)
- Корелација структуре и особина природних и синтетичких молекула и њихових комплекса са металима (RS-MESTD-Basic Research (BR or ON)-172017)
DOI: 10.1016/j.jinorgbio.2011.12.002
ISSN: 0162-0134
PubMed: 22266461
WoS: 000302205600008
Scopus: 2-s2.0-84855888296
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Gligorijević, Nevenka AU - Aranđelović, Sandra AU - Filipović, Lana AU - Jakovljević, Ksenija AU - Jankovic, Radmila AU - Grgurić-Šipka, Sanja AU - Ivanović, Ivanka AU - Radulović, Siniša AU - Tešić, Živoslav Lj. PY - 2012 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1270 AB - In our previous study, ruthenium(II)-p-cymene complexes of general formula [(eta(6)-p-cymene)Ru(L)Cl2], L: 3-acetylpyridine (1), 2-amino-5-chloropyridine (2); and [(eta(6)-p-cymene)Ru(HL)Cl], HL: 2,3-pyridinedicarboxylic acid (3), 2,4-pyridinedicarboxylic acid (4), revealed low antiproliferative activity, except complex [(eta(6)-p-cymene)RuCl(picolinic acid)]center dot H2O (5) which exhibited IC50 around 80 mu M. In this study we further investigated in vitro potential of antimetastatic action of ruthenium complexes on HeLa and two endothelial cell lines. Comparison of structure and activity of five complexes indicated heterogenic mode of activity, with regard to the potential of antimetastatic and antiproliferative effect. Replacement of substituted pyridine ligand with picolinic acid (complex 5) around Ru(II) center contributed to complex cytotoxicity and ruthenium DNA binding affinity. Analysis of ruthenium(II) accumulation in DNA and protein fractions of HeLa cells, using ICP-OES revealed significantly higher content of complex 5 in DNA fraction in comparison to the other tested compounds. It also altered cell cycle progression, affected expression of DNA repair enzymes ERCC1 and MSH2, and showed enhanced activity in combination with 3-aminobenzamide. Regardless of their effect on cell growth, Ru(II) complexes exerted antimetastatic effect on several tumor cell lines in vitro, achieved mostly by the effect on cell adhesion, migration and angiogenesis, while picolinate ruthenium(II)-arene additionally exerted inhibitory effect on extracellular matrix degradation. (c) 2011 Elsevier Inc. All rights reserved. PB - Elsevier Science Inc, New York T2 - Journal of Inorganic Biochemistry T1 - Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure VL - 108 SP - 53 EP - 61 DO - 10.1016/j.jinorgbio.2011.12.002 ER -
@article{ author = "Gligorijević, Nevenka and Aranđelović, Sandra and Filipović, Lana and Jakovljević, Ksenija and Jankovic, Radmila and Grgurić-Šipka, Sanja and Ivanović, Ivanka and Radulović, Siniša and Tešić, Živoslav Lj.", year = "2012", abstract = "In our previous study, ruthenium(II)-p-cymene complexes of general formula [(eta(6)-p-cymene)Ru(L)Cl2], L: 3-acetylpyridine (1), 2-amino-5-chloropyridine (2); and [(eta(6)-p-cymene)Ru(HL)Cl], HL: 2,3-pyridinedicarboxylic acid (3), 2,4-pyridinedicarboxylic acid (4), revealed low antiproliferative activity, except complex [(eta(6)-p-cymene)RuCl(picolinic acid)]center dot H2O (5) which exhibited IC50 around 80 mu M. In this study we further investigated in vitro potential of antimetastatic action of ruthenium complexes on HeLa and two endothelial cell lines. Comparison of structure and activity of five complexes indicated heterogenic mode of activity, with regard to the potential of antimetastatic and antiproliferative effect. Replacement of substituted pyridine ligand with picolinic acid (complex 5) around Ru(II) center contributed to complex cytotoxicity and ruthenium DNA binding affinity. Analysis of ruthenium(II) accumulation in DNA and protein fractions of HeLa cells, using ICP-OES revealed significantly higher content of complex 5 in DNA fraction in comparison to the other tested compounds. It also altered cell cycle progression, affected expression of DNA repair enzymes ERCC1 and MSH2, and showed enhanced activity in combination with 3-aminobenzamide. Regardless of their effect on cell growth, Ru(II) complexes exerted antimetastatic effect on several tumor cell lines in vitro, achieved mostly by the effect on cell adhesion, migration and angiogenesis, while picolinate ruthenium(II)-arene additionally exerted inhibitory effect on extracellular matrix degradation. (c) 2011 Elsevier Inc. All rights reserved.", publisher = "Elsevier Science Inc, New York", journal = "Journal of Inorganic Biochemistry", title = "Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure", volume = "108", pages = "53-61", doi = "10.1016/j.jinorgbio.2011.12.002" }
Gligorijević, N., Aranđelović, S., Filipović, L., Jakovljević, K., Jankovic, R., Grgurić-Šipka, S., Ivanović, I., Radulović, S.,& Tešić, Ž. Lj.. (2012). Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure. in Journal of Inorganic Biochemistry Elsevier Science Inc, New York., 108, 53-61. https://doi.org/10.1016/j.jinorgbio.2011.12.002
Gligorijević N, Aranđelović S, Filipović L, Jakovljević K, Jankovic R, Grgurić-Šipka S, Ivanović I, Radulović S, Tešić ŽL. Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure. in Journal of Inorganic Biochemistry. 2012;108:53-61. doi:10.1016/j.jinorgbio.2011.12.002 .
Gligorijević, Nevenka, Aranđelović, Sandra, Filipović, Lana, Jakovljević, Ksenija, Jankovic, Radmila, Grgurić-Šipka, Sanja, Ivanović, Ivanka, Radulović, Siniša, Tešić, Živoslav Lj., "Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure" in Journal of Inorganic Biochemistry, 108 (2012):53-61, https://doi.org/10.1016/j.jinorgbio.2011.12.002 . .