Приказ основних података о документу

dc.creatorČavić, Milena
dc.creatorGrozdanović, Milica
dc.creatorBajić, Aleksandar
dc.creatorSrdić-Rajić, Tatjana
dc.creatorAndjus, Pavle R.
dc.creatorGavrović-Jankulović, Marija
dc.date.accessioned2018-11-22T00:21:04Z
dc.date.available2018-11-22T00:21:04Z
dc.date.issued2012
dc.identifier.issn0031-9422
dc.identifier.urihttps://cherry.chem.bg.ac.rs/handle/123456789/1284
dc.description.abstractActinidin belongs to the papain-like family of cysteine proteases and is a major kiwifruit allergen. In this study, the effect of actinidin on cellular morphology and adhesion of T84 intestinal cells was investigated. Both rounding and detachment of T84 cells were observed upon actinidin treatment. The morphological changes and cell desquamation was protease-dependent, as well as time- and concentration-dependent. Changes of intercellular adhesion and adhesion of epithelial cells to collagen upon actinidin treatment could be responsible for the cell rounding and give rise to discontinuous breaches in the epithelial monolayer observed in this study. Actinidin's action on cell morphology, adhesion and monolayer integrity were not due to compromised viability of T84 epithelial cells, as confirmed by MTT assay and flow cytometric analysis of the cell cycle. Damage to the epithelial monolayer of the intestine induced by actinidin should be further evaluated as an important factor in the development of kiwifruit allergy and other intestinal disorders.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourcePhytochemistry
dc.subjectActinidia sp.en
dc.subjectKiwifruiten
dc.subjectActinidinen
dc.subjectT84 cellsen
dc.subjectIntestinal disordersen
dc.titleActinidin, a protease from kiwifruit, induces changes in morphology and adhesion of T84 intestinal epithelial cellsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБајиц, Aлександар; Гроздановиц, Милица; Aндјус, Павле Р.; Цавиц, Милена; Гавровић-Јанкуловић, Марија; Срдиц-Рајиц, Татјана;
dc.citation.volume77
dc.citation.spage46
dc.citation.epage52
dc.identifier.wos000303273300003
dc.identifier.doi10.1016/j.phytochem.2011.12.014
dc.citation.other77: 46-52
dc.citation.rankM21
dc.identifier.pmid22257750
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84859163978


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Приказ основних података о документу