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dc.creatorJovanović, Vesna B.
dc.creatorPenezić-Romanjuk, Ana Z.
dc.creatorPavicevic, Ivan D.
dc.creatorAćimović, Jelena M.
dc.creatorMandić, Ljuba M.
dc.date.accessioned2018-11-22T00:23:26Z
dc.date.available2018-11-22T00:23:26Z
dc.date.issued2013
dc.identifier.issn0003-2697
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1369
dc.description.abstractThe thiol (Cys34) content of human serum albumin (HSA-SH) decreases during oxidative and carbonyl stress and, therefore, could represent a useful parameter in clinical practice. Nevertheless, the reliability of HSA-thiol determination with Ellman's method depends on the purity of isolated HSA. Determination of total serum thiols (mmol/L) and HSA-SH content (mmol -SH/mmol HSA) after HSA isolation from diabetic patient and control sera by a two-step precipitation with ammonium sulfate (AS), as well as HSA-SH contribution (%) to total serum thiols, was assessed. Purity and yield of isolated HSA were monitored spectrophotometrically and by native polyacrylamide gel electrophoresis. Precipitation of HSA from serum via a two-step method with AS produced HSA with 91.9 +/- 3.6% purity and 69.7 +/- 4.4% yield, allowing for precise (relative standard deviation of 3.2%) and reliable (comparing with total serum thiols) measurement of HSA-SH content with DTNB [5,5'-dithiobis-(2-nitrobenzoic acid)]. The content of the HSA-SH group in patients with type 2 diabetes was significantly (P lt 0.05) lower compared with that of the healthy cohort (0.483 +/- 0.067 vs. 0.561 +/- 0.054 mmol -SH/mmol HSA). Because the proposed method of HSA isolation is simple, time-efficient, and technically less demanding, and it also enables reliable determination of HSA-SH content, it is suitable for clinical practice. (C) 2013 Elsevier Inc. All rights reserved.en
dc.publisherAcademic Press Inc Elsevier Science, San Diego
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceAnalytical Biochemistry
dc.subjectAlbumin thiol group determinationen
dc.subjectAlbumin isolationen
dc.subjectTwo-step precipitation methoden
dc.subjectDiabetesen
dc.subjectOxidative and carbonyl stressen
dc.titleImproving the reliability of human serum albumin-thiol group determinationen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПавицевиц, Иван Д.; Пенезиц-Ромањук, Aна З.; Мандић, Љуба М.; Јовановић, Весна; Aћимовић, Јелена;
dc.citation.volume439
dc.citation.issue1
dc.citation.spage17
dc.citation.epage22
dc.identifier.wos000321029200006
dc.identifier.doi10.1016/j.ab.2013.03.033
dc.citation.other439(1): 17-22
dc.citation.rankM22
dc.identifier.pmid23583909
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84877805283
dc.identifier.rcubKon_2489


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