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dc.creatorCasey, William T.
dc.creatorNikodinović-Runić, Jasmina
dc.creatorFonseca Garcia, Pilar
dc.creatorGuzik, Maciej W.
dc.creatorMcGrath, John W.
dc.creatorQuinn, John P.
dc.creatorCagney, Gerard
dc.creatorAuxiliadora Prieto, Maria
dc.creatorO'Connor, Kevin E.
dc.date.accessioned2018-11-22T00:24:04Z
dc.date.available2018-11-22T00:24:04Z
dc.date.issued2013
dc.identifier.issn1758-2229
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1408
dc.description.abstractThe primary enzyme involved in polyphosphate (polyP) synthesis, polyP kinase (ppk), has been deleted in Pseudomonas putidaKT2440. This has resulted in a threefold to sixfold reduction in polyhydroxyalkanoate (PHA) accumulation compared with the wild type under conditions of nitrogen limitation, with either temperature or oxidative (H2O2) stress, when grown on glucose. The accumulation of PHA by ppk mutant was the same as the wild type under nitrogen-limiting growth conditions. There was no difference in polyP levels between wild-type and ppk strains under all growth conditions tested. In the ppk mutant proteome, polyP kinase (PPK) was undetectable, but up-regulation of the polyp-associated proteins polyP adenosine triphosphate (ATP)/nicotinamide adenine dinucleotide (NAD) kinase (PpnK), a putative polyP adenosine monophosphate (AMP) phosphotransferase (PP_1752), and exopolyphosphatase was observed. ppk strain exhibited significantly retarded growth with glycerol as carbon and energy source (42h of lag period compared with 24h in wild-type strain) but similar growth to the wild-type strain with glucose. Analysis of gene transcription revealed downregulation of glycerol kinase and the glycerol facilitator respectively. Glycerol kinase protein expression was also downregulated in the ppk mutant. The deletion of ppk did not affect motility but reduced biofilm formation. Thus, the knockout of the ppk gene has resulted in a number of phenotypic changes to the mutant without affecting polyP accumulation.en
dc.publisherWiley-Blackwell, Hoboken
dc.rightsrestrictedAccess
dc.sourceEnvironmental Microbiology Reports
dc.titleThe effect of polyphosphate kinase gene deletion on polyhydroxyalkanoate accumulation and carbon metabolism in Pseudomonas putida KT2440en
dc.typearticle
dc.rights.licenseARR
dcterms.abstractAуxилиадора Прието, Мариа; О'Цоннор, Кевин Е.; Цасеy, Wиллиам Т.; Цагнеy, Герард; Гузик, Мациеј W.; Фонсеца Гарциа, Пилар; МцГратх, Јохн W.; Qуинн, Јохн П.; Никодиновић-Рунић, Јасмина;
dc.citation.volume5
dc.citation.issue5
dc.citation.spage740
dc.citation.epage746
dc.identifier.wos000325142700014
dc.identifier.doi10.1111/1758-2229.12076
dc.citation.other5(5): 740-746
dc.citation.rankM21
dc.identifier.pmid24115625
dc.identifier.rcubKon_2528


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