A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae
Само за регистроване кориснике
2013
Аутори
Pavlović, MarijanaDimitrijevic, A.
Trbojević-Ivić, Jovana
Milosavic, N.
Gavrović-Jankulović, Marija
Bezbradica, Dejan
Velickovic, D.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
alpha-1,4-Glucosidase from Saccharomyces cerevisiae is an enzyme which is widely used in synthesis of different drugs. Glucosidase inhibitors are studied as potential drugs for prevention of HIV and diabetes. For understanding of these processes it is very important to have insights in the transglucosylation activity of this enzyme. In this paper the kinetics of transglucosylation reaction catalyzed by this enzyme in the synthesis of benzyl alcohol glucoside was studied and all relevant kinetic constants for this system are found. It was shown one additional property of transglycosylation reactions catalyzed by glycosidases-inhibition by both, glucose acceptor and glucose donor, and mechanisms for these inhibitions were proposed.
Кључне речи:
alpha-glucosidase / transglucosylation / benzyl alcohol glucoside / substrate inhibition / ping-pong mechanisamИзвор:
Russian Journal of Physical Chemistry A, 2013, 87, 13, 2285-2288Издавач:
- Maik Nauka/Interperiodica/Springer, New York
Финансирање / пројекти:
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-172049)
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-46010)
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-256716)
DOI: 10.1134/S0036024413130207
ISSN: 0036-0244
WoS: 000326718200028
Scopus: 2-s2.0-84888146485
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Pavlović, Marijana AU - Dimitrijevic, A. AU - Trbojević-Ivić, Jovana AU - Milosavic, N. AU - Gavrović-Jankulović, Marija AU - Bezbradica, Dejan AU - Velickovic, D. PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1432 AB - alpha-1,4-Glucosidase from Saccharomyces cerevisiae is an enzyme which is widely used in synthesis of different drugs. Glucosidase inhibitors are studied as potential drugs for prevention of HIV and diabetes. For understanding of these processes it is very important to have insights in the transglucosylation activity of this enzyme. In this paper the kinetics of transglucosylation reaction catalyzed by this enzyme in the synthesis of benzyl alcohol glucoside was studied and all relevant kinetic constants for this system are found. It was shown one additional property of transglycosylation reactions catalyzed by glycosidases-inhibition by both, glucose acceptor and glucose donor, and mechanisms for these inhibitions were proposed. PB - Maik Nauka/Interperiodica/Springer, New York T2 - Russian Journal of Physical Chemistry A T1 - A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae VL - 87 IS - 13 SP - 2285 EP - 2288 DO - 10.1134/S0036024413130207 ER -
@article{ author = "Pavlović, Marijana and Dimitrijevic, A. and Trbojević-Ivić, Jovana and Milosavic, N. and Gavrović-Jankulović, Marija and Bezbradica, Dejan and Velickovic, D.", year = "2013", abstract = "alpha-1,4-Glucosidase from Saccharomyces cerevisiae is an enzyme which is widely used in synthesis of different drugs. Glucosidase inhibitors are studied as potential drugs for prevention of HIV and diabetes. For understanding of these processes it is very important to have insights in the transglucosylation activity of this enzyme. In this paper the kinetics of transglucosylation reaction catalyzed by this enzyme in the synthesis of benzyl alcohol glucoside was studied and all relevant kinetic constants for this system are found. It was shown one additional property of transglycosylation reactions catalyzed by glycosidases-inhibition by both, glucose acceptor and glucose donor, and mechanisms for these inhibitions were proposed.", publisher = "Maik Nauka/Interperiodica/Springer, New York", journal = "Russian Journal of Physical Chemistry A", title = "A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae", volume = "87", number = "13", pages = "2285-2288", doi = "10.1134/S0036024413130207" }
Pavlović, M., Dimitrijevic, A., Trbojević-Ivić, J., Milosavic, N., Gavrović-Jankulović, M., Bezbradica, D.,& Velickovic, D.. (2013). A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae. in Russian Journal of Physical Chemistry A Maik Nauka/Interperiodica/Springer, New York., 87(13), 2285-2288. https://doi.org/10.1134/S0036024413130207
Pavlović M, Dimitrijevic A, Trbojević-Ivić J, Milosavic N, Gavrović-Jankulović M, Bezbradica D, Velickovic D. A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae. in Russian Journal of Physical Chemistry A. 2013;87(13):2285-2288. doi:10.1134/S0036024413130207 .
Pavlović, Marijana, Dimitrijevic, A., Trbojević-Ivić, Jovana, Milosavic, N., Gavrović-Jankulović, Marija, Bezbradica, Dejan, Velickovic, D., "A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae" in Russian Journal of Physical Chemistry A, 87, no. 13 (2013):2285-2288, https://doi.org/10.1134/S0036024413130207 . .