Repozitorijum Hemijskog fakulteta - Cherry
Univerzitet u Beogradu - Hemijski fakultet
    • English
    • Српски
    • Српски (Serbia)
  • Srpski (latinica) 
    • Engleski
    • Srpski (ćirilica)
    • Srpski (latinica)
  • Prijava
Pregled rada 
  •   Repozitorijum Hemijskog fakulteta - Cherry
  • Hemijski fakultet
  • Radovi
  • Pregled rada
  •   Repozitorijum Hemijskog fakulteta - Cherry
  • Hemijski fakultet
  • Radovi
  • Pregled rada
JavaScript is disabled for your browser. Some features of this site may not work without it.

FACS Based High Throughput Screening Systems for Gene Libraries in Double Emulsions

Nema prikaza
Autori
Prodanović, Radivoje
Ostafe, Raluca
Blanusa, Milan
Schwaneberg, Ulrich
Konferencijski prilog
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
A flow cytometry based high throughput screening system for glucose oxidase (GOx) gene libraries in double emulsions was developed. Firstly, encapsulation of yeast cells in double emulsion was optimized by changing the ABIL EM90 concentration in light mineral oil from 2.9% to 1.5%. This enabled formation of larger water droplets and more efficient yeast cell encapsulation. Several fluorescent assays for hydrogen peroxide were tested and the 3-carboxy-7-(4'-aminophenoxy)-coumarine (APCC) oxidation by horseradish peroxidase based assay best fit the requirements of the double emulsion technology. Using an optimized substrate solution consisting of 0.5 mM APCC, 40 mM glucose and 10 U/mL of horse radish peroxidase, a referent gene library containing 10(7) yeast cells was sorted in 30 min and enriched from 1% to 15% of yeast cells expressing wt GOx.
Izvor:
Progress in Colloid and Polymer Science, 2012, 139, 51-
Izdavač:
  • Springer-Verlag Berlin, Berlin
Projekti:
  • company BRAIN AG
  • Alexander von Humboldt foundation
  • BMBF BiochancePlus program

DOI: 10.1007/978-3-642-28974-3_10

ISSN: 0340-255X

WoS: 000310177800010

Scopus: 2-s2.0-84870692826
[ Google Scholar ]
URI
http://cherry.chem.bg.ac.rs/handle/123456789/1542
Kolekcije
  • Radovi
Institucija
Hemijski fakultet

DSpace software copyright © 2002-2015  DuraSpace
O Cherry repozitorijumu | Pošaljite zapažanja

OpenAIRERCUB
 

 

Kompletan repozitorijumInstitucijeAutoriNasloviTemeOva institucijaAutoriNasloviTeme

DSpace software copyright © 2002-2015  DuraSpace
O Cherry repozitorijumu | Pošaljite zapažanja

OpenAIRERCUB