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dc.creatorProdanović, Radivoje
dc.creatorOstafe, Raluca
dc.creatorBlanusa, Milan
dc.creatorSchwaneberg, Ulrich
dc.date.accessioned2018-11-22T00:22:08Z
dc.date.available2018-11-22T00:22:08Z
dc.date.issued2012
dc.identifier.issn0340-255X
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1542
dc.description.abstractA flow cytometry based high throughput screening system for glucose oxidase (GOx) gene libraries in double emulsions was developed. Firstly, encapsulation of yeast cells in double emulsion was optimized by changing the ABIL EM90 concentration in light mineral oil from 2.9% to 1.5%. This enabled formation of larger water droplets and more efficient yeast cell encapsulation. Several fluorescent assays for hydrogen peroxide were tested and the 3-carboxy-7-(4'-aminophenoxy)-coumarine (APCC) oxidation by horseradish peroxidase based assay best fit the requirements of the double emulsion technology. Using an optimized substrate solution consisting of 0.5 mM APCC, 40 mM glucose and 10 U/mL of horse radish peroxidase, a referent gene library containing 10(7) yeast cells was sorted in 30 min and enriched from 1% to 15% of yeast cells expressing wt GOx.en
dc.publisherSpringer-Verlag Berlin, Berlin
dc.relationcompany BRAIN AG
dc.relationAlexander von Humboldt foundation
dc.relationBMBF BiochancePlus program
dc.rightsrestrictedAccess
dc.sourceProgress in Colloid and Polymer Science
dc.titleFACS Based High Throughput Screening Systems for Gene Libraries in Double Emulsionsen
dc.typeconferenceObject
dc.rights.licenseARR
dcterms.abstractПродановић, Радивоје; Блануса, Милан; Остафе, Ралуца; Сцхwанеберг, Улрицх;
dc.citation.volume139
dc.citation.spage51
dc.identifier.wos000310177800010
dc.identifier.doi10.1007/978-3-642-28974-3_10
dc.citation.other139: 51-57
dc.identifier.scopus2-s2.0-84870692826
dc.identifier.rcubKon_2373


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