Show simple item record

dc.creatorStankovic, Nada
dc.creatorRadulovic, Vanja
dc.creatorPetkovic, Milos
dc.creatorVučković, Ivan M.
dc.creatorJadranin, Milka
dc.creatorVasiljevic, Branka
dc.creatorNikodinović-Runić, Jasmina
dc.date.accessioned2018-11-22T00:22:14Z
dc.date.available2018-11-22T00:22:14Z
dc.date.issued2012
dc.identifier.issn0175-7598
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1548
dc.description.abstractA Gram-positive, red-pigment-producing bacterial strain, designated JS520 was isolated from the pristine sediment from the cave on mountain Miroc in Serbia. Strain was confirmed to belong to Streptomyces genus based on phenotypic and genetic analysis. Streptomyces sp. JS520 has the ability to produce exceptionally high amounts of deep red pigment into both solid and liquid media. Liquid chromatography and mass spectroscopy of the purified pigments revealed the major component to be undecylprodigiosin (93 %) with minor component being oxidatively cyclized derivative. The pigment production was affected by medium composition, temperature, pH, and the aeration rate. By medium optimization, yields of undecylprodigiosin of 138 mg l(-1) were achieved, what is the highest level of undecylprodigiosin production reported for the members of Gram-positive Streptomyces genus. Purified pigment had antimicrobial properties against bacterial Bacillus and Micrococcus species (50 mu g ml(-1)) and against Candida albicans species (100-200 mu g ml(-1) range). The ability to affect auto-oxidation of the linoleic acid was demonstrated for the purified undecylprodigiosin, suggesting antioxidative properties of this pigment. Multiple ecophysiological roles of the pigment were revealed by comparing cultures grown under pigment-producing and pigment-nonproducing conditions. Cells grown under undecylprodigiosin-producing conditions could tolerate presence of hydrogen peroxide exhibiting three times smaller zones of inhibition at 100 mM H2O2. Undecylprodigiosin-producing cells were also less susceptible to tetracycline, kanamycin, chloramphenicol, and 8-hydroxyquinoline. While the growth of the cells not producing pigment was completely inhibited by 15 min of exposure to ultraviolet light (254 nm), cells producing undecylprodigiosin and cells supplied with purified pigment in vitro showed survival rates at 22 and 8 %, respectively.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173048/RS//
dc.rightsrestrictedAccess
dc.sourceApplied Microbiology and Biotechnology
dc.subjectPigmenten
dc.subjectUndecylprodigiosinen
dc.subjectAntioxidativeen
dc.subjectUV protectionen
dc.subjectStreptomycesen
dc.subjectMedium optimizationen
dc.titleStreptomyces sp JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective propertiesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСтанковиц, Нада; Васиљевиц, Бранка; Петковиц, Милос; Вуцковиц, Иван; Радуловиц, Вања; Јадранин, Милка; Никодиновић-Рунић, Јасмина;
dc.citation.volume96
dc.citation.issue5
dc.citation.spage1217
dc.citation.epage1231
dc.identifier.wos000310866800009
dc.identifier.doi10.1007/s00253-012-4237-3
dc.citation.other96(5): 1217-1231
dc.citation.rankM21
dc.identifier.pmid22767180
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84871050546
dc.identifier.rcubKon_2379


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record