Immobilization of periodate oxidized invertase by adsorption on sepiolite

Abstract

Periodate oxidized invertase was immobilized by adsorption on sepiolite. The obtained immobilized enzyme was more resistant to washing out by concentrated salt solution. and had an eight times higher half-life at 60 degreesC than adsorbed native invertase. In packed bed reactor 50%, conversion of 500 g/dm(3) sucrose at 40 degreesC and a flow rate of 1 bv/h was achieved. The specific productivity of the immobilized invertase was 0.187 kg/dm(3)/h.

Perjodatno oksidovana invertaza je imobilizovana adsorpcijom na sepiolitu. Dobijeni imobilizovani enzim je bio rezistentniji na ispiranje koncentrovanim rastvorima soli i imao je osam puta veći poluživot na 60 ºC od adsorbovane nativne invertaze. U protočnom cevastom reaktoru dobili smo 50 % konverziju rastvora saharoze koncentracije 500 g/dm3 na 40 ºC i pri protoku od 1 bv/h. Specifična produktivnost imobilizovane invertaze je bila 0,187 kg/dm3/h.