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dc.creatorVujčić, Zoran
dc.creatorJanović, Barbara
dc.creatorLončar, Nikola
dc.creatorMargetic, Aleksandra
dc.creatorBozic, Natasa
dc.creatorDojnov, Biljana
dc.creatorVujčić, Miroslava
dc.date.accessioned2018-11-22T00:30:30Z
dc.date.available2018-11-22T00:30:30Z
dc.date.issued2015
dc.identifier.issn0964-8305
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1644
dc.description.abstractHorseradish peroxidase (HRP) is enzyme first described more than 200 years ago and yet there are still some aspects of this potent enzyme to be tackled. Researchers were focused on most abundant isoenzyme HRP CIA while remaining, particularly anionic isoenzymes were discarded in purification process. This work describes exploitation of those isoenzymes for removal of recalcitrant pollutants such as reactive dyes. Results demonstrated that not only these enzymes can decolorize dyes but also in some cases anionic forms are more efficient than commercially produced cationic HRP form. Enzyme concentration of 0.14 U ml(-1) was found to provide maximum dye removal at optimized reaction conditions with dye concentration of 30 mg I-1. Majority of dyes tested were successfully decolorized at pH 5 or 7 while some dyes like Orange 2 and Reactive black 5 are decolorized most efficiently at pH 9. Anionic isoenzymes act by disrupting chromophore of Reactive black 5 while cationic HRP oxidize dye but leaves chromophore present. (C) 2014 Elsevier Ltd. All rights reserved.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsrestrictedAccess
dc.sourceInternational Biodeterioration and Biodegradation
dc.subjectHorseradish peroxidaseen
dc.subjectHRPen
dc.subjectDecolorizationen
dc.subjectReactive dyesen
dc.subjectZymogramen
dc.subjectIzoenzymesen
dc.titleExploitation of neglected horseradish peroxidase izoenzymes for dye decolorizationen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВујциц, Мирослава; Бозиц, Натаса; Лончар, Никола; Јановиц, Барбара; Дојнов, Биљана; Вујчић, Зоран; Маргетиц, Aлександра;
dc.citation.volume97
dc.citation.spage124
dc.citation.epage127
dc.identifier.wos000348557700016
dc.identifier.doi10.1016/j.ibiod.2014.10.007
dc.citation.other97: 124-127
dc.citation.rankM22~
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84912567704
dc.identifier.rcubKon_2790


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