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dc.creatorMisic, Danijela
dc.creatorSiler, Branislav
dc.creatorGašić, Uroš M.
dc.creatorAvramov, Stevan N.
dc.creatorZivkovic, Suzana
dc.creatorZivkovic, Jasmina Nestorovic
dc.creatorMilutinovic, Milica
dc.creatorTešić, Živoslav Lj.
dc.date.accessioned2018-11-22T00:30:43Z
dc.date.available2018-11-22T00:30:43Z
dc.date.issued2015
dc.identifier.issn0958-0344
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1663
dc.description.abstractIntroduction - Nepeta species contain a variety of secondary metabolites, including iridoid monoterpenes - nepetalactones and phenolic acids - that are considered the main bioactive constituents. This work represents the first attempt to comparatively explore variations in these two major groups of secondary metabolites within the genus. Objective - To develop an efficient analytical methodology for simultaneous analysis of nepetalactones and phenolic acids in methanol extracts of selected Nepeta species, and to evaluate its potential application in chemotaxonomic studies. Material and methods - A UHPLC combined with linear-trap quadrupole (LTQ) orbitrap MS method was used to characterise chemical diversity and complexity of phenolics among 12 selected Nepeta species. A targeted metabolomic approach using UHPLC coupled to a diode array detector (DAD) and combined with (+/-) heated electrospray ionisation (HESI) MS/MS was developed and validated for quantitative analysis of six hydroxycinnamic acid derivatives and four nepetalactones. Results - Phenolic profiling provided a valuable database of bioactive compounds in the plant group studied, including phenolic acids (hydroxybenzoic and hydroxycinnamic acids) and flavonoids (flavones, flavonols and flavanones). Principal component analysis and cluster analysis suggested the applicability of 10 targeted compounds as chemomarkers for chemotaxonomic studies. Pearson's correlation analysis revealed significant positive correlations between metabolites involved in different biosynthetic pathways (phenylpropanoid or monoterpenoid). Conclusion - The described targeted metabolomic approach proved to be highly beneficial in designing a phytochemical overview of the genus Nepeta, and might have applications in further clarification of phylogenetic relations. Furthermore, it has the potential to be implemented in a routine quality control of plant material and herbal preparations. Copyright (C) 2014 John Wiley & Sons, Ltd.en
dc.publisherWiley-Blackwell, Hoboken
dc.rightsrestrictedAccess
dc.sourcePhytochemical Analysis
dc.subjectChemotaxonomyen
dc.subjecttargeted metabolomic analysisen
dc.subjectnepetalactoneen
dc.subjectphenolic acidsen
dc.subjectcatminten
dc.subjectcatnipen
dc.subjectLamiaceaeen
dc.subjectNepetaen
dc.titleSimultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studiesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractТешић, Живослав Љ.; Силер, Бранислав; Зивковиц, Јасмина Несторовиц; Зивковиц, Сузана; Aврамов, Стеван; Мисиц, Данијела; Милутиновиц, Милица; Гашић, Урош М.;
dc.citation.volume26
dc.citation.issue1
dc.citation.spage72
dc.citation.epage85
dc.identifier.wos000349963000010
dc.identifier.doi10.1002/pca.2538
dc.citation.other26(1): 72-85
dc.citation.rankM21~
dc.identifier.pmid25431035
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84917691460
dc.identifier.rcubKon_2809


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