Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10
Само за регистроване кориснике
2015
Аутори
Ilić-Tomić, TatjanaGenčić, Marija S.
Živković, Miodrag V.
Vasiljević, Branka
Đokić, Lidija
Nikodinović-Runić, Jasmina
Radulović, Niko S.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 A degrees C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C-7-C-30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with anoth...er soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.
Кључне речи:
Streptomyces sp NP10 / Lipidomics / Free fatty acids / Branched cyclopropane fatty acids / Ecophysiological role / Microbial biodiesel productionИзвор:
Applied Microbiology and Biotechnology, 2015, 99, 11, 4815-4833Издавач:
- Springer, New York
Финансирање / пројекти:
- Комбинаторне библиотеке хетерогених катализатора, природних производа, модификованих природних производа и њихових аналога: пут ка новим биолошки активним агенсима (RS-MESTD-Basic Research (BR or ON)-172061)
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
Напомена:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3430
DOI: 10.1007/s00253-014-6364-5
ISSN: 0175-7598
PubMed: 25636833
WoS: 000354617800024
Scopus: 2-s2.0-84939960802
Колекције
Институција/група
Inovacioni centar / Innovation CentreTY - JOUR AU - Ilić-Tomić, Tatjana AU - Genčić, Marija S. AU - Živković, Miodrag V. AU - Vasiljević, Branka AU - Đokić, Lidija AU - Nikodinović-Runić, Jasmina AU - Radulović, Niko S. PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1713 AB - Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 A degrees C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C-7-C-30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production. PB - Springer, New York T2 - Applied Microbiology and Biotechnology T1 - Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10 VL - 99 IS - 11 SP - 4815 EP - 4833 DO - 10.1007/s00253-014-6364-5 ER -
@article{ author = "Ilić-Tomić, Tatjana and Genčić, Marija S. and Živković, Miodrag V. and Vasiljević, Branka and Đokić, Lidija and Nikodinović-Runić, Jasmina and Radulović, Niko S.", year = "2015", abstract = "Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 A degrees C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C-7-C-30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.", publisher = "Springer, New York", journal = "Applied Microbiology and Biotechnology", title = "Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10", volume = "99", number = "11", pages = "4815-4833", doi = "10.1007/s00253-014-6364-5" }
Ilić-Tomić, T., Genčić, M. S., Živković, M. V., Vasiljević, B., Đokić, L., Nikodinović-Runić, J.,& Radulović, N. S.. (2015). Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10. in Applied Microbiology and Biotechnology Springer, New York., 99(11), 4815-4833. https://doi.org/10.1007/s00253-014-6364-5
Ilić-Tomić T, Genčić MS, Živković MV, Vasiljević B, Đokić L, Nikodinović-Runić J, Radulović NS. Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10. in Applied Microbiology and Biotechnology. 2015;99(11):4815-4833. doi:10.1007/s00253-014-6364-5 .
Ilić-Tomić, Tatjana, Genčić, Marija S., Živković, Miodrag V., Vasiljević, Branka, Đokić, Lidija, Nikodinović-Runić, Jasmina, Radulović, Niko S., "Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10" in Applied Microbiology and Biotechnology, 99, no. 11 (2015):4815-4833, https://doi.org/10.1007/s00253-014-6364-5 . .