Production and properties of glucoamylase from Aspergillus niger WT
Produkcija i osobine glukoamilaze iz divljeg soja Aspergillus niger
Apstrakt
Aspergillus niger WT was cultivated in a liquid medium containing corn meal, on two types of shakers. Measured glucoamylase production reached maximum after 96 h. When the organism was grown on a rotary shaker it produced two times more enzyme than when it was grown on a reciprocal shaker. Studies of glucoamylase characteristics revealed that the optimum temperature for its activity was 65 oC. Half life of the obtained enzyme was 120 min at 60 oC. The optimum pH of the enzyme was 4.5. A procedure for detecting the activity of glucoamylase on a isoelectric focusing (IEF) gel by KI reagent was described. Since the method is very sensitive, it will be useful for enzyme engineering studies that involve screening of various mutations.
Aspergillus niger WT je gajen u tečnom medijumu koji sadrži kukuruzni griz na dva tipa mućkalica. Produkcija glukoamilaze dostigla je maksimum posle 96h. Kada se organizam gaji na rotacionoj mućkalici produkcija enzima je dva puta veća nego kada se gaji na recipročnoj mućkalici. Proučavanje karakteristika glukoamilaze pokazalo je da je temperaturni optimum za njenu aktivnost 65 oC. Poluživot dobijenog enzima je 120 min na 60 oC. pH optimum enzima je 4,5. U radu je opisana i procedura za detektovanje aktivnosti glukoamilaze u gelu nakon IEF koristeći KI reagens. Metoda je veoma osetljiva i može se koristiti za proučavanje strukture enzima skriningom različitih mutacija.
Ključne reči:
Aspergillus niger / glucoamylase / zymogramIzvor:
Acta biologica iugoslavica - serija B: Mikrobiologija, 2001, 38, 2, 71-78Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Milosavić, Nenad AU - Prodanović, Radivoje AU - Vujčić, Zoran PY - 2001 UR - https://cherry.chem.bg.ac.rs/handle/123456789/174 AB - Aspergillus niger WT was cultivated in a liquid medium containing corn meal, on two types of shakers. Measured glucoamylase production reached maximum after 96 h. When the organism was grown on a rotary shaker it produced two times more enzyme than when it was grown on a reciprocal shaker. Studies of glucoamylase characteristics revealed that the optimum temperature for its activity was 65 oC. Half life of the obtained enzyme was 120 min at 60 oC. The optimum pH of the enzyme was 4.5. A procedure for detecting the activity of glucoamylase on a isoelectric focusing (IEF) gel by KI reagent was described. Since the method is very sensitive, it will be useful for enzyme engineering studies that involve screening of various mutations. AB - Aspergillus niger WT je gajen u tečnom medijumu koji sadrži kukuruzni griz na dva tipa mućkalica. Produkcija glukoamilaze dostigla je maksimum posle 96h. Kada se organizam gaji na rotacionoj mućkalici produkcija enzima je dva puta veća nego kada se gaji na recipročnoj mućkalici. Proučavanje karakteristika glukoamilaze pokazalo je da je temperaturni optimum za njenu aktivnost 65 oC. Poluživot dobijenog enzima je 120 min na 60 oC. pH optimum enzima je 4,5. U radu je opisana i procedura za detektovanje aktivnosti glukoamilaze u gelu nakon IEF koristeći KI reagens. Metoda je veoma osetljiva i može se koristiti za proučavanje strukture enzima skriningom različitih mutacija. T2 - Acta biologica iugoslavica - serija B: Mikrobiologija T1 - Production and properties of glucoamylase from Aspergillus niger WT T1 - Produkcija i osobine glukoamilaze iz divljeg soja Aspergillus niger VL - 38 IS - 2 SP - 71 EP - 78 UR - https://hdl.handle.net/21.15107/rcub_cherry_174 ER -
@article{ author = "Milosavić, Nenad and Prodanović, Radivoje and Vujčić, Zoran", year = "2001", abstract = "Aspergillus niger WT was cultivated in a liquid medium containing corn meal, on two types of shakers. Measured glucoamylase production reached maximum after 96 h. When the organism was grown on a rotary shaker it produced two times more enzyme than when it was grown on a reciprocal shaker. Studies of glucoamylase characteristics revealed that the optimum temperature for its activity was 65 oC. Half life of the obtained enzyme was 120 min at 60 oC. The optimum pH of the enzyme was 4.5. A procedure for detecting the activity of glucoamylase on a isoelectric focusing (IEF) gel by KI reagent was described. Since the method is very sensitive, it will be useful for enzyme engineering studies that involve screening of various mutations., Aspergillus niger WT je gajen u tečnom medijumu koji sadrži kukuruzni griz na dva tipa mućkalica. Produkcija glukoamilaze dostigla je maksimum posle 96h. Kada se organizam gaji na rotacionoj mućkalici produkcija enzima je dva puta veća nego kada se gaji na recipročnoj mućkalici. Proučavanje karakteristika glukoamilaze pokazalo je da je temperaturni optimum za njenu aktivnost 65 oC. Poluživot dobijenog enzima je 120 min na 60 oC. pH optimum enzima je 4,5. U radu je opisana i procedura za detektovanje aktivnosti glukoamilaze u gelu nakon IEF koristeći KI reagens. Metoda je veoma osetljiva i može se koristiti za proučavanje strukture enzima skriningom različitih mutacija.", journal = "Acta biologica iugoslavica - serija B: Mikrobiologija", title = "Production and properties of glucoamylase from Aspergillus niger WT, Produkcija i osobine glukoamilaze iz divljeg soja Aspergillus niger", volume = "38", number = "2", pages = "71-78", url = "https://hdl.handle.net/21.15107/rcub_cherry_174" }
Milosavić, N., Prodanović, R.,& Vujčić, Z.. (2001). Production and properties of glucoamylase from Aspergillus niger WT. in Acta biologica iugoslavica - serija B: Mikrobiologija, 38(2), 71-78. https://hdl.handle.net/21.15107/rcub_cherry_174
Milosavić N, Prodanović R, Vujčić Z. Production and properties of glucoamylase from Aspergillus niger WT. in Acta biologica iugoslavica - serija B: Mikrobiologija. 2001;38(2):71-78. https://hdl.handle.net/21.15107/rcub_cherry_174 .
Milosavić, Nenad, Prodanović, Radivoje, Vujčić, Zoran, "Production and properties of glucoamylase from Aspergillus niger WT" in Acta biologica iugoslavica - serija B: Mikrobiologija, 38, no. 2 (2001):71-78, https://hdl.handle.net/21.15107/rcub_cherry_174 .