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dc.creatorSavić, Aleksandar
dc.creatorMisirlic-Dencic, Sonja
dc.creatorDulovic, Marija
dc.creatorMihajlović-Lalić, Ljiljana
dc.creatorJovanovic, Maja
dc.creatorGrgurić-Šipka, Sanja
dc.creatorMarković, Ivanka
dc.creatorSabo, Tibor
dc.date.accessioned2018-11-22T00:28:31Z
dc.date.available2018-11-22T00:28:31Z
dc.date.issued2014
dc.identifier.issn0045-2068
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1811
dc.description.abstractThis study involves the synthesis and characterization of novel cyclohexyl 1,3-propanediamine-N, N'-diacetate molecules as well as investigation of their cytotoxic action. New acid 1a was synthesized by reaction between (S)-2-amino-3-cyclohexylpropanoic acid and 1,3-dibromopropane, while the esters (1b-1e) derived from this acid were obtained by reaction of the corresponding absolute alcohol, thionyl chloride and synthesized acid. All compounds were characterized by IR, ESI-MS, (H-1, C-13 and HSQC) NMR spectroscopy and elemental analysis. The cytotoxic activity of all compounds was tested on several tumour cell lines: human (U251) and rat (C6) glioma, human promyelocytic leukaemia (HL-60), human neuroblastoma (SHSY-5Y) and mouse fibrosarcoma (L929) as well as primary rat astrocytes. The present study reveals potent antitumour activity of novel purely organic compounds (1a-1e), which was most pronounced in human glioma (U251) cells. The esterification is required for the novel compounds' cytotoxic action since the n-butyl ester 1e was the most efficient compound. Importantly, n-butyl ester 1e was more toxic to glioma cells in comparison to rat astrocytes, with 24-h IC50 values lower than those for cisplatin. n-Butyl ester 1e induced production of reactive oxygen species (ROS) and caused an oxidative- stress-derived accumulation of glioma cells in the G(0)/G(1) phase of the cell cycle, as well as caspase activation and DNA fragmentation, suggesting that apoptosis induction plays an important role in the novel compounds' antiglioma action. (C) 2014 Elsevier Inc. All rights reserved.en
dc.publisherAcademic Press Inc Elsevier Science, San Diego
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172035/RS//
dc.rightsrestrictedAccess
dc.sourceBioorganic Chemistry
dc.subjectAmine ligandsen
dc.subjectApoptosisen
dc.subjectn-Butyl esteren
dc.subjectCyclohexyl analoguesen
dc.subjectCytotoxic activityen
dc.subjectOxidative stressen
dc.titleSynthesis, characterization and ROS-mediated cytotoxic action of novel (S, S)-1,3-propanediamine-N,N '-di-2-(3-cyclohexyl)propanoic acid and corresponding estersen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМисирлиц-Денциц, Соња; Савић, Aлександар; Марковиц, Иванка; Михајловић-Лалић, Љиљана; Гргурић-Шипка, Сања; Сабо, Тибор; Јовановиц, Маја; Дуловиц, Марија;
dc.citation.volume54
dc.citation.spage73
dc.citation.epage80
dc.identifier.wos000338732900011
dc.identifier.doi10.1016/j.bioorg.2014.04.006
dc.citation.other54: 73-80
dc.citation.rankM22
dc.identifier.pmid24836201
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84901287105
dc.identifier.rcubKon_2694


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