Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex
Само за регистроване кориснике
2014
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A novel collagenolytic serine protease was identified and then purified (along with ficin) to apparent homogeneity from the latex of fig (Ficus carica, var. Brown Turkey) by two step chromatographic procedure using gel and covalent chromatography. The enzyme is a monomeric protein of molecular mass of 41 +/- 9 kDa as estimated by analytical gel filtration chromatography. It is an acidic protein with a pI value of approximately 5 and optimal activity at pH 8.0-8.5 and temperature 60 degrees C. The enzymatic activity was strongly inhibited by PMSF and Pefabloc SC, indicating that the enzyme is a serine protease. The enzyme showed specificity towards gelatin and collagen (215 GDU/mg and 24.8 CDU/mg, respectively) and non-specific protease activity (0.18 U/mg against casein). The enzyme was stable and retained full activity over a broad range of pH and temperature. The fig latex collagenolytic protease is potentially useful as a non-microbial enzyme with collagenolytic activity for various... applications in the fields of biochemistry, biotechnology and medicine. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
Кључне речи:
Collagenolytic activity / Gelatinolytic activity / Serine protease / Fig latex / Enzyme purificationИзвор:
Journal of Bioscience and Bioengineering, 2014, 118, 6, 622-627Издавач:
- Soc Bioscience Bioengineering Japan, Osaka
Финансирање / пројекти:
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-MESTD-Basic Research (BR or ON)-172049)
DOI: 10.1016/j.jbiosc.2014.05.020
ISSN: 1389-1723
PubMed: 24982021
WoS: 000347869100003
Scopus: 2-s2.0-84922819521
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Rašković, Brankica AU - Bozovic, Olga AU - Prodanović, Radivoje AU - Niketić, Vesna AU - Polović, Natalija PY - 2014 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1902 AB - A novel collagenolytic serine protease was identified and then purified (along with ficin) to apparent homogeneity from the latex of fig (Ficus carica, var. Brown Turkey) by two step chromatographic procedure using gel and covalent chromatography. The enzyme is a monomeric protein of molecular mass of 41 +/- 9 kDa as estimated by analytical gel filtration chromatography. It is an acidic protein with a pI value of approximately 5 and optimal activity at pH 8.0-8.5 and temperature 60 degrees C. The enzymatic activity was strongly inhibited by PMSF and Pefabloc SC, indicating that the enzyme is a serine protease. The enzyme showed specificity towards gelatin and collagen (215 GDU/mg and 24.8 CDU/mg, respectively) and non-specific protease activity (0.18 U/mg against casein). The enzyme was stable and retained full activity over a broad range of pH and temperature. The fig latex collagenolytic protease is potentially useful as a non-microbial enzyme with collagenolytic activity for various applications in the fields of biochemistry, biotechnology and medicine. (C) 2014, The Society for Biotechnology, Japan. All rights reserved. PB - Soc Bioscience Bioengineering Japan, Osaka T2 - Journal of Bioscience and Bioengineering T1 - Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex VL - 118 IS - 6 SP - 622 EP - 627 DO - 10.1016/j.jbiosc.2014.05.020 ER -
@article{ author = "Rašković, Brankica and Bozovic, Olga and Prodanović, Radivoje and Niketić, Vesna and Polović, Natalija", year = "2014", abstract = "A novel collagenolytic serine protease was identified and then purified (along with ficin) to apparent homogeneity from the latex of fig (Ficus carica, var. Brown Turkey) by two step chromatographic procedure using gel and covalent chromatography. The enzyme is a monomeric protein of molecular mass of 41 +/- 9 kDa as estimated by analytical gel filtration chromatography. It is an acidic protein with a pI value of approximately 5 and optimal activity at pH 8.0-8.5 and temperature 60 degrees C. The enzymatic activity was strongly inhibited by PMSF and Pefabloc SC, indicating that the enzyme is a serine protease. The enzyme showed specificity towards gelatin and collagen (215 GDU/mg and 24.8 CDU/mg, respectively) and non-specific protease activity (0.18 U/mg against casein). The enzyme was stable and retained full activity over a broad range of pH and temperature. The fig latex collagenolytic protease is potentially useful as a non-microbial enzyme with collagenolytic activity for various applications in the fields of biochemistry, biotechnology and medicine. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.", publisher = "Soc Bioscience Bioengineering Japan, Osaka", journal = "Journal of Bioscience and Bioengineering", title = "Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex", volume = "118", number = "6", pages = "622-627", doi = "10.1016/j.jbiosc.2014.05.020" }
Rašković, B., Bozovic, O., Prodanović, R., Niketić, V.,& Polović, N.. (2014). Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex. in Journal of Bioscience and Bioengineering Soc Bioscience Bioengineering Japan, Osaka., 118(6), 622-627. https://doi.org/10.1016/j.jbiosc.2014.05.020
Rašković B, Bozovic O, Prodanović R, Niketić V, Polović N. Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex. in Journal of Bioscience and Bioengineering. 2014;118(6):622-627. doi:10.1016/j.jbiosc.2014.05.020 .
Rašković, Brankica, Bozovic, Olga, Prodanović, Radivoje, Niketić, Vesna, Polović, Natalija, "Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex" in Journal of Bioscience and Bioengineering, 118, no. 6 (2014):622-627, https://doi.org/10.1016/j.jbiosc.2014.05.020 . .