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dc.creatorDojnov, Biljana
dc.creatorGrujic, Marica
dc.creatorVujčić, Zoran
dc.date.accessioned2018-11-22T00:33:18Z
dc.date.available2018-11-22T00:33:18Z
dc.date.issued2015
dc.identifier.issn0352-5139
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/2011
dc.description.abstractTriticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry.en
dc.publisherSerbian Chemical Soc, Belgrade
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsopenAccess
dc.sourceJournal of the Serbian Chemical Society
dc.subjectalpha-amylaseen
dc.subjectglucoamylaseen
dc.subjectfungien
dc.subjectenzyme productionen
dc.subjectsolid-state fermentationen
dc.subjectraw starchen
dc.titleHighly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrateen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractВујчић, Зоран; Дојнов, Биљана; Грујиц, Марица;
dc.citation.volume80
dc.citation.issue11
dc.citation.spage1375
dc.citation.epage1390
dc.identifier.wos000366585800004
dc.identifier.doi10.2298/JSC150317041D
dc.citation.other80(11): 1375-1390
dc.citation.rankM23
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-84957599434
dc.identifier.rcubKon_2966


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Приказ основних података о документу