Identification of Seed Coat Phenolic Compounds from Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity
2015
Autori
Stanisavljević, Nemanja S.Ilić, Marija D.
Jovanović, Živko S.
Čupić, Tihomir
Dabić Zagorac, Dragana
Natić, Maja
Tešić, Živoslav Lj.
Radovic, Svetlana S.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antiox...idant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.
Ključne reči:
Pisum sativum L. / seed coat / UHPLC-LTQ OrbiTrap MS / phenolic content / antioxidantIzvor:
Archives of biological sciences, 2015, 67, 3, 829-840Izdavač:
- Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
Finansiranje / projekti:
- Molekularni mehanizmi odgovora biljaka na abiotički stres-uloga transkripcionih faktora i malih RNK i analiza genetičkog diverziteta biljnih kultura od interesa za poljoprivredu i biotehnologiju (RS-173005)
- Korelacija strukture i osobina prirodnih i sintetičkih molekula i njihovih kompleksa sa metalima (RS-172017)
DOI: 10.2298/ABS141204042S
ISSN: 0354-4664
WoS: 000366794800008
Scopus: 2-s2.0-84950117705
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Stanisavljević, Nemanja S. AU - Ilić, Marija D. AU - Jovanović, Živko S. AU - Čupić, Tihomir AU - Dabić Zagorac, Dragana AU - Natić, Maja AU - Tešić, Živoslav Lj. AU - Radovic, Svetlana S. PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2013 AB - The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products. PB - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd T2 - Archives of biological sciences T1 - Identification of Seed Coat Phenolic Compounds from Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity VL - 67 IS - 3 SP - 829 EP - 840 DO - 10.2298/ABS141204042S ER -
@article{ author = "Stanisavljević, Nemanja S. and Ilić, Marija D. and Jovanović, Živko S. and Čupić, Tihomir and Dabić Zagorac, Dragana and Natić, Maja and Tešić, Živoslav Lj. and Radovic, Svetlana S.", year = "2015", abstract = "The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.", publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd", journal = "Archives of biological sciences", title = "Identification of Seed Coat Phenolic Compounds from Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity", volume = "67", number = "3", pages = "829-840", doi = "10.2298/ABS141204042S" }
Stanisavljević, N. S., Ilić, M. D., Jovanović, Ž. S., Čupić, T., Dabić Zagorac, D., Natić, M., Tešić, Ž. Lj.,& Radovic, S. S.. (2015). Identification of Seed Coat Phenolic Compounds from Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity. in Archives of biological sciences Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 67(3), 829-840. https://doi.org/10.2298/ABS141204042S
Stanisavljević NS, Ilić MD, Jovanović ŽS, Čupić T, Dabić Zagorac D, Natić M, Tešić ŽL, Radovic SS. Identification of Seed Coat Phenolic Compounds from Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity. in Archives of biological sciences. 2015;67(3):829-840. doi:10.2298/ABS141204042S .
Stanisavljević, Nemanja S., Ilić, Marija D., Jovanović, Živko S., Čupić, Tihomir, Dabić Zagorac, Dragana, Natić, Maja, Tešić, Živoslav Lj., Radovic, Svetlana S., "Identification of Seed Coat Phenolic Compounds from Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity" in Archives of biological sciences, 67, no. 3 (2015):829-840, https://doi.org/10.2298/ABS141204042S . .