Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)
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Popović, Milica M.Sustar, Vid
Gricar, Jozica
Straus, Ines
Torkar, Gregor
Kraigher, Hojka
de Marco, Ario
Article (Published version)
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Climate development models predict alterations that will critically influence plant metabolism in southern and central Europe. Although the molecular players involved in the response to climatic stress factors have been well described in crops, little information is available for forest tree species. Consequently, the identification of molecular biomarkers suitable for evaluating the actual impact of different environmental stress conditions on forest plants would be of great importance for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of temperature stress in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the combined analysis of the specific activities and isoform profile of peroxidases, superoxide dismutases, and glutathione peroxidases coupled wit...h the amount variation of phenolic compounds enabled the discrimination between stressed and control seedlings. This approach represents a promising platform for the assessment of temperature stress in forest trees and could also enhance selection and breeding practices, allowing for plants more tolerant and (or) resistant to abiotic stress.
Keywords:
abiotic stress / enzyme activity / forestry / peroxidase / temperature stressSource:
Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere, 2016, 46, 1, 58-66Publisher:
- Canadian Science Publishing, Nrc Research Press, Ottawa
Funding / projects:
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)
- [L4-2262]
- Slovenian ARRS research programme Forest Biology, Ecology and Technology [P4-0107]
- [L404318]
Note:
- Peer-reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/3530
DOI: 10.1139/cjfr-2015-0274
ISSN: 0045-5067
WoS: 000370049100007
Scopus: 2-s2.0-84953400926
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Popović, Milica M. AU - Sustar, Vid AU - Gricar, Jozica AU - Straus, Ines AU - Torkar, Gregor AU - Kraigher, Hojka AU - de Marco, Ario PY - 2016 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2049 AB - Climate development models predict alterations that will critically influence plant metabolism in southern and central Europe. Although the molecular players involved in the response to climatic stress factors have been well described in crops, little information is available for forest tree species. Consequently, the identification of molecular biomarkers suitable for evaluating the actual impact of different environmental stress conditions on forest plants would be of great importance for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of temperature stress in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the combined analysis of the specific activities and isoform profile of peroxidases, superoxide dismutases, and glutathione peroxidases coupled with the amount variation of phenolic compounds enabled the discrimination between stressed and control seedlings. This approach represents a promising platform for the assessment of temperature stress in forest trees and could also enhance selection and breeding practices, allowing for plants more tolerant and (or) resistant to abiotic stress. PB - Canadian Science Publishing, Nrc Research Press, Ottawa T2 - Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere T1 - Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris) VL - 46 IS - 1 SP - 58 EP - 66 DO - 10.1139/cjfr-2015-0274 ER -
@article{ author = "Popović, Milica M. and Sustar, Vid and Gricar, Jozica and Straus, Ines and Torkar, Gregor and Kraigher, Hojka and de Marco, Ario", year = "2016", abstract = "Climate development models predict alterations that will critically influence plant metabolism in southern and central Europe. Although the molecular players involved in the response to climatic stress factors have been well described in crops, little information is available for forest tree species. Consequently, the identification of molecular biomarkers suitable for evaluating the actual impact of different environmental stress conditions on forest plants would be of great importance for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of temperature stress in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the combined analysis of the specific activities and isoform profile of peroxidases, superoxide dismutases, and glutathione peroxidases coupled with the amount variation of phenolic compounds enabled the discrimination between stressed and control seedlings. This approach represents a promising platform for the assessment of temperature stress in forest trees and could also enhance selection and breeding practices, allowing for plants more tolerant and (or) resistant to abiotic stress.", publisher = "Canadian Science Publishing, Nrc Research Press, Ottawa", journal = "Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere", title = "Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)", volume = "46", number = "1", pages = "58-66", doi = "10.1139/cjfr-2015-0274" }
Popović, M. M., Sustar, V., Gricar, J., Straus, I., Torkar, G., Kraigher, H.,& de Marco, A.. (2016). Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere Canadian Science Publishing, Nrc Research Press, Ottawa., 46(1), 58-66. https://doi.org/10.1139/cjfr-2015-0274
Popović MM, Sustar V, Gricar J, Straus I, Torkar G, Kraigher H, de Marco A. Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere. 2016;46(1):58-66. doi:10.1139/cjfr-2015-0274 .
Popović, Milica M., Sustar, Vid, Gricar, Jozica, Straus, Ines, Torkar, Gregor, Kraigher, Hojka, de Marco, Ario, "Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)" in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere, 46, no. 1 (2016):58-66, https://doi.org/10.1139/cjfr-2015-0274 . .