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Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents

Samo za registrovane korisnike
2018
Autori
Vukojević, Vesna
Đurđić, Slađana Z.
Šovrc, Ľubomír
Ćirković-Veličković, Tanja
Mutić, Jelena
Stanković, Dalibor
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with sati...sfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.

Ključne reči:
Ergosterol / Boron-doped diamond electrode / Mushrooms
Izvor:
Food Analytical Methods, 2018, 11, 9, 2590-2596
Izdavač:
  • Springer, New York
Projekti:
  • Primena unapređenih oksidacionih procesa i nanostrukturisanih oksidnih materijala za uklanjanje zagađivača iz životne sredine, razvoj i optimizacija instrumentalnih tehnika za praćenje efikasnosti (RS-172030)
  • Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-621375)
  • Grant Agency of the Slovak Republic [1/0489/16]

DOI: 10.1007/s12161-018-1249-3

ISSN: 1936-9751

WoS: 000438995800026

Scopus: 2-s2.0-85045073042
[ Google Scholar ]
2
2
URI
http://cherry.chem.bg.ac.rs/handle/123456789/2184
Kolekcije
  • Radovi
  • Radovi
Institucija
Hemijski fakultet

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