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dc.creatorVukojević, Vesna
dc.creatorĐurđić, Slađana Z.
dc.creatorŠovrc, Ľubomír
dc.creatorĆirković-Veličković, Tanja
dc.creatorMutić, Jelena
dc.creatorStanković, Dalibor
dc.date.accessioned2018-11-22T00:44:45Z
dc.date.available2018-11-22T00:44:45Z
dc.date.issued2018
dc.identifier.issn1936-9751
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/2184
dc.description.abstractErgosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172030/RS//
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/621375/EU//
dc.relationGrant Agency of the Slovak Republic [1/0489/16]
dc.rightsrestrictedAccess
dc.sourceFood Analytical Methods
dc.subjectErgosterolen
dc.subjectBoron-doped diamond electrodeen
dc.subjectMushroomsen
dc.titleAnalytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solventsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСворц, Лубомир; Вукојевић, Весна; Ђурђић, Слађана; Ћирковић-Величковић, Тања; Мутић, Јелена; Станковић, Далибор;
dc.citation.volume11
dc.citation.issue9
dc.citation.spage2590
dc.citation.epage2596
dc.identifier.wos000438995800026
dc.identifier.doi10.1007/s12161-018-1249-3
dc.citation.other11(9): 2590-2596
dc.citation.rankM21a
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-85045073042
dc.identifier.rcubKon_3515


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