Development of RAST assay for determination of anti-Populus canadensis IgE antibodies

Abstract

One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.