Antileukemic action of novel diamine Pt(II) halogenido complexes: Comparison of the representative novel Pt(II) with corresponding Pt(IV) complex
Само за регистроване кориснике
2017
Аутори
Misirlić-Denčić, SonjaPoljarević, Jelena
Isaković, Anđelka M.
Marković, Ivanka
Sabo, Tibor
Grgurić-Šipka, Sanja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study presents the synthesis, characterization, and antitumor action of five new Pt(II) halogenido, chlorido, and iodido complexes with edda type of ligands. (S,S)-Ethylenediamine-N,N-di-2-(3-cyclohexyl)propanoic acid dihydrochloride and its methyl, ethyl, and n-propyl esters were prepared according to the previously reported procedure. All investigated complexes were characterized by IR, ESI-MS (H-1, C-13, and HMBC) NMR spectroscopy, and elemental analysis. Their cytotoxic action was investigated in four human tumor cell lines: promyelocytic (HL-60) and lymphocytic (REH) leukemia, glioma (U251), and lung carcinoma (H460). Cell viability was assessed by acid phosphatase and LDH assay, while oxidative stress and cell death parameters were analyzed by flow cytometry. The results showed that novel Pt(II) complexes exhibited antitumor action superior to precursor ligands, with iodido complexes being more efficient than corresponding chlorido complexes. Human promyelocytic cell line (H...L-60) was the most sensitive to antitumor action of all investigated substances and was used for investigation of the underlying mode of antileukemic action. The investigated Pt(II) complexes showed more potent antileukemic action than corresponding Pt(IV) complex, through induction of oxidative stress and apoptosis, evidenced by caspase (8, 9, and 3) activation and phosphatidylserine externalization.
Кључне речи:
antileukemic / caspases / edda ligands / phosphatidylserine / platinum(II) complexesИзвор:
Chemical Biology and Drug Design, 2017, 90, 2, 262-271Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Рационални дизајн и синтеза биолошки активних и координационих једињења и функционалних материјала, релевантних у (био)нанотехнологији (RS-MESTD-Basic Research (BR or ON)-172035)
- Модулација сигналних путева који контролишу интрацелуларни енергетски баланс у терапији тумора и неуро-имуно-ендокриних поремећаја (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41025)
Напомена:
- Peer-reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/3120
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3121
DOI: 10.1111/cbdd.12945
ISSN: 1747-0277
PubMed: 28102932
WoS: 000405102900010
Scopus: 2-s2.0-85013104444
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Misirlić-Denčić, Sonja AU - Poljarević, Jelena AU - Isaković, Anđelka M. AU - Marković, Ivanka AU - Sabo, Tibor AU - Grgurić-Šipka, Sanja PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2483 AB - This study presents the synthesis, characterization, and antitumor action of five new Pt(II) halogenido, chlorido, and iodido complexes with edda type of ligands. (S,S)-Ethylenediamine-N,N-di-2-(3-cyclohexyl)propanoic acid dihydrochloride and its methyl, ethyl, and n-propyl esters were prepared according to the previously reported procedure. All investigated complexes were characterized by IR, ESI-MS (H-1, C-13, and HMBC) NMR spectroscopy, and elemental analysis. Their cytotoxic action was investigated in four human tumor cell lines: promyelocytic (HL-60) and lymphocytic (REH) leukemia, glioma (U251), and lung carcinoma (H460). Cell viability was assessed by acid phosphatase and LDH assay, while oxidative stress and cell death parameters were analyzed by flow cytometry. The results showed that novel Pt(II) complexes exhibited antitumor action superior to precursor ligands, with iodido complexes being more efficient than corresponding chlorido complexes. Human promyelocytic cell line (HL-60) was the most sensitive to antitumor action of all investigated substances and was used for investigation of the underlying mode of antileukemic action. The investigated Pt(II) complexes showed more potent antileukemic action than corresponding Pt(IV) complex, through induction of oxidative stress and apoptosis, evidenced by caspase (8, 9, and 3) activation and phosphatidylserine externalization. PB - Wiley, Hoboken T2 - Chemical Biology and Drug Design T1 - Antileukemic action of novel diamine Pt(II) halogenido complexes: Comparison of the representative novel Pt(II) with corresponding Pt(IV) complex VL - 90 IS - 2 SP - 262 EP - 271 DO - 10.1111/cbdd.12945 ER -
@article{ author = "Misirlić-Denčić, Sonja and Poljarević, Jelena and Isaković, Anđelka M. and Marković, Ivanka and Sabo, Tibor and Grgurić-Šipka, Sanja", year = "2017", abstract = "This study presents the synthesis, characterization, and antitumor action of five new Pt(II) halogenido, chlorido, and iodido complexes with edda type of ligands. (S,S)-Ethylenediamine-N,N-di-2-(3-cyclohexyl)propanoic acid dihydrochloride and its methyl, ethyl, and n-propyl esters were prepared according to the previously reported procedure. All investigated complexes were characterized by IR, ESI-MS (H-1, C-13, and HMBC) NMR spectroscopy, and elemental analysis. Their cytotoxic action was investigated in four human tumor cell lines: promyelocytic (HL-60) and lymphocytic (REH) leukemia, glioma (U251), and lung carcinoma (H460). Cell viability was assessed by acid phosphatase and LDH assay, while oxidative stress and cell death parameters were analyzed by flow cytometry. The results showed that novel Pt(II) complexes exhibited antitumor action superior to precursor ligands, with iodido complexes being more efficient than corresponding chlorido complexes. Human promyelocytic cell line (HL-60) was the most sensitive to antitumor action of all investigated substances and was used for investigation of the underlying mode of antileukemic action. The investigated Pt(II) complexes showed more potent antileukemic action than corresponding Pt(IV) complex, through induction of oxidative stress and apoptosis, evidenced by caspase (8, 9, and 3) activation and phosphatidylserine externalization.", publisher = "Wiley, Hoboken", journal = "Chemical Biology and Drug Design", title = "Antileukemic action of novel diamine Pt(II) halogenido complexes: Comparison of the representative novel Pt(II) with corresponding Pt(IV) complex", volume = "90", number = "2", pages = "262-271", doi = "10.1111/cbdd.12945" }
Misirlić-Denčić, S., Poljarević, J., Isaković, A. M., Marković, I., Sabo, T.,& Grgurić-Šipka, S.. (2017). Antileukemic action of novel diamine Pt(II) halogenido complexes: Comparison of the representative novel Pt(II) with corresponding Pt(IV) complex. in Chemical Biology and Drug Design Wiley, Hoboken., 90(2), 262-271. https://doi.org/10.1111/cbdd.12945
Misirlić-Denčić S, Poljarević J, Isaković AM, Marković I, Sabo T, Grgurić-Šipka S. Antileukemic action of novel diamine Pt(II) halogenido complexes: Comparison of the representative novel Pt(II) with corresponding Pt(IV) complex. in Chemical Biology and Drug Design. 2017;90(2):262-271. doi:10.1111/cbdd.12945 .
Misirlić-Denčić, Sonja, Poljarević, Jelena, Isaković, Anđelka M., Marković, Ivanka, Sabo, Tibor, Grgurić-Šipka, Sanja, "Antileukemic action of novel diamine Pt(II) halogenido complexes: Comparison of the representative novel Pt(II) with corresponding Pt(IV) complex" in Chemical Biology and Drug Design, 90, no. 2 (2017):262-271, https://doi.org/10.1111/cbdd.12945 . .