Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan
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2017
Authors
Djurić, AleksandraGojgić-Cvijović, Gordana D.
Jakovljević, Dragica M.
Kekez, Branka
Stefanović Kojić, Jovana R.
Mattinen, Maija-Liisa
Harju, Inka Elina
Vrvić, Miroslav M.
Beškoski, Vladimir
Article (Published version)
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Show full item recordAbstract
Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NM...R) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan. (C) 2017 Elsevier B.V. All rights reserved.
Keywords:
Polluted environments / Exopolysaccharides / Brachybacterium paraconglomeratumSource:
International Journal of Biological Macromolecules, 2017, 104, 311-321Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
- FCUB ERA
- Japan International Cooperation Agency (JICA)
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/2965
DOI: 10.1016/j.ijbiomac.2017.06.034
ISSN: 0141-8130
PubMed: 28602989
WoS: 000412959300035
Scopus: 2-s2.0-85020640842
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Djurić, Aleksandra AU - Gojgić-Cvijović, Gordana D. AU - Jakovljević, Dragica M. AU - Kekez, Branka AU - Stefanović Kojić, Jovana R. AU - Mattinen, Maija-Liisa AU - Harju, Inka Elina AU - Vrvić, Miroslav M. AU - Beškoski, Vladimir PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2538 AB - Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan. (C) 2017 Elsevier B.V. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - International Journal of Biological Macromolecules T1 - Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan VL - 104 SP - 311 EP - 321 DO - 10.1016/j.ijbiomac.2017.06.034 ER -
@article{ author = "Djurić, Aleksandra and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Kekez, Branka and Stefanović Kojić, Jovana R. and Mattinen, Maija-Liisa and Harju, Inka Elina and Vrvić, Miroslav M. and Beškoski, Vladimir", year = "2017", abstract = "Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan. (C) 2017 Elsevier B.V. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "International Journal of Biological Macromolecules", title = "Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan", volume = "104", pages = "311-321", doi = "10.1016/j.ijbiomac.2017.06.034" }
Djurić, A., Gojgić-Cvijović, G. D., Jakovljević, D. M., Kekez, B., Stefanović Kojić, J. R., Mattinen, M., Harju, I. E., Vrvić, M. M.,& Beškoski, V.. (2017). Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. in International Journal of Biological Macromolecules Elsevier Science Bv, Amsterdam., 104, 311-321. https://doi.org/10.1016/j.ijbiomac.2017.06.034
Djurić A, Gojgić-Cvijović GD, Jakovljević DM, Kekez B, Stefanović Kojić JR, Mattinen M, Harju IE, Vrvić MM, Beškoski V. Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. in International Journal of Biological Macromolecules. 2017;104:311-321. doi:10.1016/j.ijbiomac.2017.06.034 .
Djurić, Aleksandra, Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Kekez, Branka, Stefanović Kojić, Jovana R., Mattinen, Maija-Liisa, Harju, Inka Elina, Vrvić, Miroslav M., Beškoski, Vladimir, "Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan" in International Journal of Biological Macromolecules, 104 (2017):311-321, https://doi.org/10.1016/j.ijbiomac.2017.06.034 . .