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dc.creatorLozo, Jelena
dc.creatorMirković, Nemanja
dc.creatorO'Connor, Paula M.
dc.creatorMalešević, Milka
dc.creatorMiljković, Marija
dc.creatorPolović, Natalija
dc.creatorJovčić, Branko
dc.creatorCotter, Paul D.
dc.creatorKojić, Milan O.
dc.date.accessioned2018-11-22T00:41:24Z
dc.date.available2018-11-22T00:41:24Z
dc.date.issued2017
dc.identifier.issn0099-2240
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/2541
dc.description.abstractLactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A. IMPORTANCE Lactolisterin BU, a broad-spectrum leaderless bacteriocin produced by L. lactis subsp. lactis bv. diacetylactis BGBU1-4, expresses strong antimicrobial activity against food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU showed the highest similarity to aureocin-like bacteriocins produced by different bacteria. The operon for synthesis is located on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4, indicating possible horizontal transfer among producers.en
dc.publisherAmer Soc Microbiology, Washington
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//
dc.rightsopenAccess
dc.sourceApplied and Environmental Microbiology
dc.subjectbacteriocinen
dc.subjectlactolisterin BUen
dc.subjectantilisterial activityen
dc.titleLactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4en
dc.typearticle
dc.rights.licenseARR
dcterms.abstractО'Цоннор, Паула М.; Цоттер, Паул Д.; Малесевиц, Милка; Мирковиц, Немања; Миљковиц, Марија; Половић, Наталија; Јовциц, Бранко; Којиц, Милан; Лозо, Јелена;
dc.citation.volume83
dc.citation.issue21
dc.identifier.wos000413104500013
dc.identifier.doi10.1128/AEM.01519-17
dc.citation.other83(21):
dc.citation.rankM21
dc.identifier.pmid28842543
dc.description.otherSupplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2999]
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85031687597
dc.identifier.fulltexthttp://cherry.chem.bg.ac.rs/bitstream/id/9483/2539.pdf
dc.identifier.rcubKon_3357


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