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Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium
dc.creator | Gojgić-Cvijović, Gordana D. | |
dc.creator | Jakovljević, Dragica M. | |
dc.creator | Lončarević, Branka D. | |
dc.creator | Todorović, Nevena M. | |
dc.creator | Pergal, Marija V. | |
dc.creator | Ćirić, J. | |
dc.creator | Loos, K. | |
dc.creator | Beškoski, Vladimir | |
dc.creator | Vrvić, Miroslav M. | |
dc.date.accessioned | 2018-12-24T08:56:48Z | |
dc.date.available | 2019-10-03 | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0141-8130 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/2799 | |
dc.description.abstract | The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose. © 2018 Elsevier B.V. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172053/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172062/RS// | |
dc.rights | embargoedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | International Journal of Biological Macromolecules | |
dc.subject | Levan | en |
dc.subject | Molasses | en |
dc.subject | Molecular weight | en |
dc.title | Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Пергал, М.В.; Цириц, Ј.; Лоос, К.; Гојгиц-Цвијовиц, Г.Д.; Јаковљевиц, Д.М.; Лонцаревиц, Б.Д.; Тодоровиц, Н.М.; Бешкоски, Владимир; Врвиц, М.М.; | |
dc.citation.volume | 121 | |
dc.citation.spage | 142 | |
dc.citation.epage | 151 | |
dc.identifier.wos | 000452346100018 | |
dc.identifier.doi | 10.1016/j.ijbiomac.2018.10.019 | |
dc.citation.other | 121: 142-151 | |
dc.citation.rank | aM21 | |
dc.description.other | Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2943] | |
dc.description.other | This is peer reviewed version of the following article: Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Loncarevic, B. D.; Todorovic, N. M.; Pergal, M. V.; Ciric, J.; Loos, K.; Beškoski, V.; Vrvić, M. M. Production of Levan by Bacillus Licheniformis NS032 in Sugar Beet Molasses-Based Medium. International Journal of Biological Macromolecules 2019, 121, 142–151. [https://doi.org/10.1016/j.ijbiomac.2018.10.019] | |
dc.type.version | acceptedVersion | en |
dc.identifier.scopus | 2-s2.0-85054437248 | |
dc.identifier.fulltext | https://cherry.chem.bg.ac.rs/bitstream/id/6404/10.1016@j.ijbiomac.2018.10.019.pdf |