Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes
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The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damagi...ng effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential. (C) 2016 Elsevier B.V. All rights reserved.
Кључне речи:
Comet assay / Decolorization / DNA damage / DNA interactions / Dye degradation / Horseradish peroxidase aИзвор:
Journal of Hazardous Materials, 2017, 321, 576-585Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Производња, изоловање и карактеризација ензима и малих молекула и њихова примена у растворном и имобилизованом облику у биотехнологији хране, биогоривима и заштитити животне средине (RS-172048)
- FEBS Collaborative Experimental Scholarship, Oslo, Norway
Напомена:
- This is the peer-reviewed version of the following article: Janović, B. S.; Collins, A. R.; Vujčić, Z. M.; Vujčić, M. T. Acidic Horseradish Peroxidase Activity Abolishes Genotoxicity of Common Dyes. Journal of Hazardous Materials 2017, 321, 576–585. https://doi.org/10.1016/j.jhazmat.2016.09.037
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3190
DOI: 10.1016/j.jhazmat.2016.09.037
ISSN: 0304-3894
WoS: 000388777300061
Scopus: 2-s2.0-84988963283
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Janović, Barbara AU - Collins, Andrew R. AU - Vujčić, Zoran AU - Vujčić, Miroslava PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3189 AB - The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential. (C) 2016 Elsevier B.V. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Hazardous Materials T1 - Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes VL - 321 SP - 576 EP - 585 DO - 10.1016/j.jhazmat.2016.09.037 ER -
@article{ author = "Janović, Barbara and Collins, Andrew R. and Vujčić, Zoran and Vujčić, Miroslava", year = "2017", abstract = "The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential. (C) 2016 Elsevier B.V. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Hazardous Materials", title = "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes", volume = "321", pages = "576-585", doi = "10.1016/j.jhazmat.2016.09.037" }
Janović, B., Collins, A. R., Vujčić, Z.,& Vujčić, M.. (2017). Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. in Journal of Hazardous Materials Elsevier Science Bv, Amsterdam., 321, 576-585. https://doi.org/10.1016/j.jhazmat.2016.09.037
Janović B, Collins AR, Vujčić Z, Vujčić M. Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. in Journal of Hazardous Materials. 2017;321:576-585. doi:10.1016/j.jhazmat.2016.09.037 .
Janović, Barbara, Collins, Andrew R., Vujčić, Zoran, Vujčić, Miroslava, "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes" in Journal of Hazardous Materials, 321 (2017):576-585, https://doi.org/10.1016/j.jhazmat.2016.09.037 . .