Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models
Нема приказа
Аутори
Kolarević, StoimirMilovanović, D.
Kračun-Kolarević, M.
Kostić, J.
Sunjog, K.
Martinović, R.
Popović-Đorđević, Jelena
Novaković, Irena T.
Sladić, Dušan
Vuković-Gačić, Branka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3′-methoxyavarone, 4′-(methylamino)avarone and 3′-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3′-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3′-methoxyavarone and 3′-(methylamino)avarone have ...a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.
Кључне речи:
Avarol / avarone / comet assay / genotoxicity / mutagenicity / SOS/umuCИзвор:
Drug and Chemical Toxicology, 2017, 1-10Издавач:
- Taylor & Francis Ltd
Напомена:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3033
DOI: 10.1080/01480545.2017.1413108
ISSN: 0148-0545
WoS: 000459818600003
Scopus: 2-s2.0-85041102861
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Kolarević, Stoimir AU - Milovanović, D. AU - Kračun-Kolarević, M. AU - Kostić, J. AU - Sunjog, K. AU - Martinović, R. AU - Popović-Đorđević, Jelena AU - Novaković, Irena T. AU - Sladić, Dušan AU - Vuković-Gačić, Branka PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/322 AB - In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3′-methoxyavarone, 4′-(methylamino)avarone and 3′-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3′-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3′-methoxyavarone and 3′-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells. PB - Taylor & Francis Ltd T2 - Drug and Chemical Toxicology T1 - Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models SP - 1 EP - 10 DO - 10.1080/01480545.2017.1413108 ER -
@article{ author = "Kolarević, Stoimir and Milovanović, D. and Kračun-Kolarević, M. and Kostić, J. and Sunjog, K. and Martinović, R. and Popović-Đorđević, Jelena and Novaković, Irena T. and Sladić, Dušan and Vuković-Gačić, Branka", year = "2017", abstract = "In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3′-methoxyavarone, 4′-(methylamino)avarone and 3′-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3′-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3′-methoxyavarone and 3′-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.", publisher = "Taylor & Francis Ltd", journal = "Drug and Chemical Toxicology", title = "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models", pages = "1-10", doi = "10.1080/01480545.2017.1413108" }
Kolarević, S., Milovanović, D., Kračun-Kolarević, M., Kostić, J., Sunjog, K., Martinović, R., Popović-Đorđević, J., Novaković, I. T., Sladić, D.,& Vuković-Gačić, B.. (2017). Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology Taylor & Francis Ltd., 1-10. https://doi.org/10.1080/01480545.2017.1413108
Kolarević S, Milovanović D, Kračun-Kolarević M, Kostić J, Sunjog K, Martinović R, Popović-Đorđević J, Novaković IT, Sladić D, Vuković-Gačić B. Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology. 2017;:1-10. doi:10.1080/01480545.2017.1413108 .
Kolarević, Stoimir, Milovanović, D., Kračun-Kolarević, M., Kostić, J., Sunjog, K., Martinović, R., Popović-Đorđević, Jelena, Novaković, Irena T., Sladić, Dušan, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models" in Drug and Chemical Toxicology (2017):1-10, https://doi.org/10.1080/01480545.2017.1413108 . .