Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex
Apstrakt
A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.
Ključne reči:
beta -Glucosidase / Cellulase / Endocellulase / Exocellulase / SSF / Trichoderma / ZymogramIzvor:
Electrophoresis, 2015, 36, 15, 1724-1727Izdavač:
- Wiley-Blackwell, Hoboken
Finansiranje / projekti:
- Proizvodnja, izolovanje i karakterizacija enzima i malih molekula i njihova primena u rastvornom i imobilizovanom obliku u biotehnologiji hrane, biogorivima i zaštititi životne sredine (RS-MESTD-Basic Research (BR or ON)-172048)
Napomena:
- This is the peer-reviewed version of the following article: Dojnov, B.; Grujić, M.; Vujčić, Z. Reliable Simultaneous Zymographic Method of Characterization of Cellulolytic Enzymes from Fungal Cellulase Complex. Electrophoresis 2015, 36 (15), 1724–1727. https://doi.org/10.1002/elps.201400541
DOI: 10.1002/elps.201400541
ISSN: 0173-0835
PubMed: 25873475
WoS: 000359057600008
Scopus: 2-s2.0-84938380974
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Dojnov, Biljana AU - Grujić, Marica AU - Vujčić, Zoran PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3408 AB - A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates. PB - Wiley-Blackwell, Hoboken T2 - Electrophoresis T1 - Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex VL - 36 IS - 15 SP - 1724 EP - 1727 DO - 10.1002/elps.201400541 ER -
@article{ author = "Dojnov, Biljana and Grujić, Marica and Vujčić, Zoran", year = "2015", abstract = "A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.", publisher = "Wiley-Blackwell, Hoboken", journal = "Electrophoresis", title = "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex", volume = "36", number = "15", pages = "1724-1727", doi = "10.1002/elps.201400541" }
Dojnov, B., Grujić, M.,& Vujčić, Z.. (2015). Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. in Electrophoresis Wiley-Blackwell, Hoboken., 36(15), 1724-1727. https://doi.org/10.1002/elps.201400541
Dojnov B, Grujić M, Vujčić Z. Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. in Electrophoresis. 2015;36(15):1724-1727. doi:10.1002/elps.201400541 .
Dojnov, Biljana, Grujić, Marica, Vujčić, Zoran, "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex" in Electrophoresis, 36, no. 15 (2015):1724-1727, https://doi.org/10.1002/elps.201400541 . .