SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives
Само за регистроване кориснике
2020
Аутори
Nešić, Maja D.Dučić, Tanja
Liang, Xinyue
Algarra, Manuel
Mi, Lan
Korićanac, Lela
Žakula, Jelena
Kop, Tatjana
Bjelaković, Mira S.
Mitrović, Aleksandra D.
Gojgić-Cvijović, Gordana D.
Stepić, Milutin
Petković, Marijana
Чланак у часопису (Објављена верзија)
,
Elsevier
Метаподаци
Приказ свих података о документуАпстракт
Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide Iband, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these fin...dings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.
Кључне речи:
Pullulan-C60 / Levan-C60 / Synchrotron radiation FTIR spectro-microscopyИзвор:
International Journal of Biological Macromolecules, 2020, 165, B, 2541-2549Издавач:
- Elsevier
Финансирање / пројекти:
- Agência Regional para o Desenvolvimento da Investigação, Tecnologia e Inovação (M1420-01-0145-FEDER-000005)
- Ministry of Science and Technology of the People's Republic of China (SINO-SERBIA2018002)
- Madeira 14-20 Program (RAM-M1420-01-0145-FEDER-000008)
- Serbian-Chinese bilateral project (451-00-478/2018-09/16)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-200017)
- ALBA Synchrotron (Grant No. 2019093770)
- Fundação para a Ciência e a Tecnologia (PEstOE/QUI/UI0674/2019)
- Fundação para a Ciência e a Tecnologia (INNOINDIGO/0001/2015)
- Fundação para a Ciência e a Tecnologia (UID/MAT/00006/2019)
- Ministerio de Ciencia, Innovación y Universidades (RTI2018-099668-BC22)
- Junta de Andalucía and FEDER funds (UMA18-FEDERJA-126)
DOI: 10.1016/j.ijbiomac.2020.10.141
ISSN: 0141-8130
WoS: 000600773500088
Scopus: 2-s2.0-85094207129
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Nešić, Maja D. AU - Dučić, Tanja AU - Liang, Xinyue AU - Algarra, Manuel AU - Mi, Lan AU - Korićanac, Lela AU - Žakula, Jelena AU - Kop, Tatjana AU - Bjelaković, Mira S. AU - Mitrović, Aleksandra D. AU - Gojgić-Cvijović, Gordana D. AU - Stepić, Milutin AU - Petković, Marijana PY - 2020 UR - https://cherry.chem.bg.ac.rs/handle/123456789/4268 AB - Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide Iband, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells. PB - Elsevier T2 - International Journal of Biological Macromolecules T1 - SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives VL - 165 IS - B SP - 2541 EP - 2549 DO - 10.1016/j.ijbiomac.2020.10.141 ER -
@article{ author = "Nešić, Maja D. and Dučić, Tanja and Liang, Xinyue and Algarra, Manuel and Mi, Lan and Korićanac, Lela and Žakula, Jelena and Kop, Tatjana and Bjelaković, Mira S. and Mitrović, Aleksandra D. and Gojgić-Cvijović, Gordana D. and Stepić, Milutin and Petković, Marijana", year = "2020", abstract = "Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide Iband, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.", publisher = "Elsevier", journal = "International Journal of Biological Macromolecules", title = "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives", volume = "165", number = "B", pages = "2541-2549", doi = "10.1016/j.ijbiomac.2020.10.141" }
Nešić, M. D., Dučić, T., Liang, X., Algarra, M., Mi, L., Korićanac, L., Žakula, J., Kop, T., Bjelaković, M. S., Mitrović, A. D., Gojgić-Cvijović, G. D., Stepić, M.,& Petković, M.. (2020). SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules Elsevier., 165(B), 2541-2549. https://doi.org/10.1016/j.ijbiomac.2020.10.141
Nešić MD, Dučić T, Liang X, Algarra M, Mi L, Korićanac L, Žakula J, Kop T, Bjelaković MS, Mitrović AD, Gojgić-Cvijović GD, Stepić M, Petković M. SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules. 2020;165(B):2541-2549. doi:10.1016/j.ijbiomac.2020.10.141 .
Nešić, Maja D., Dučić, Tanja, Liang, Xinyue, Algarra, Manuel, Mi, Lan, Korićanac, Lela, Žakula, Jelena, Kop, Tatjana, Bjelaković, Mira S., Mitrović, Aleksandra D., Gojgić-Cvijović, Gordana D., Stepić, Milutin, Petković, Marijana, "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives" in International Journal of Biological Macromolecules, 165, no. B (2020):2541-2549, https://doi.org/10.1016/j.ijbiomac.2020.10.141 . .