Evaluation of antitumor potential of Cu(II) complex with hydrazone of 2-acetylthiazole and Girard’s T reagent
Аутори
Stevanović, NevenaJevtović, Mima
Mitić, Dragana
Matić, Ivana Z.
Crnogorac, Marija Đ.
Vujčić, Miroslava
Sladić, Dušan
Čobeljić, Božidar
Anđelković, Katarina K.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this paper, the previously synthesized Cu(II) complex ([CuL1(N3) (CH3OH)]BF4) with N,N,N-trimethyl-2-oxo-2-(2-(1-(thiazol-2-yl)ethylidene)hydrazinyl)ethan-1-aminium chloride, has been characterized and its biological activity has been studied in detail. The Cu(II) complex consists of ligand coordinated in a deprotonated, formally neutral zwitter-ionic form, via NNO atoms, one azido ligand and one methanol molecule. The Cu(II) complex was selected due to results of the cytotoxic activity, the brine shrimp test and DPPH radical scavenging activity, which were previously performed. The effects of Cu(II) complex on cell cycle phase distribution of cervical adenocarcinoma HeLa cells were investigated in order to examine the mechanisms of its anticancer activity. The measurement of intracellular ROS levels in HeLa and HaCaT cell lines were evaluated in order to explore their possible generation and the role in cytotoxic activity. The possible anti-invasive and anti-angiogenic properties o...f Cu(II) complex were evaluated. DNA binding experiments, including fluorescence displacement study and DNA cleavage experiments, were performed in order to obtain information on the type of DNA-metal complex interactions. © 2022 Serbian Chemical Society. All rights reserved.
Кључне речи:
cell cycle analysis / gene expression analyses / N-acylhydrazone / UV-Vis measurementsИзвор:
Journal of the Serbian Chemical Society, 2022, 87, 2, 181-192Издавач:
- Beograd : Srpsko hemijsko društvo
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200043 (Институт за онкологију и радиологију Србије, Београд) (RS-200043)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-200168)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200288 (Иновациони центар Хемијског факултета у Београду доо) (RS-200288)
Напомена:
- Suplementary material: https://cherry.chem.bg.ac.rs/handle/123456789/5155
Повезане информације:
DOI: 10.2298/JSC211203114S
ISSN: 0352-5139
WoS: 000751730600001
Scopus: 2-s2.0-85126719693
URI
http://www.doiserbia.nb.rs/img/doi/0352-5139/2022/0352-51392202181S.pdfhttp://cherry.chem.bg.ac.rs/handle/123456789/5154
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Stevanović, Nevena AU - Jevtović, Mima AU - Mitić, Dragana AU - Matić, Ivana Z. AU - Crnogorac, Marija Đ. AU - Vujčić, Miroslava AU - Sladić, Dušan AU - Čobeljić, Božidar AU - Anđelković, Katarina K. PY - 2022 UR - http://www.doiserbia.nb.rs/img/doi/0352-5139/2022/0352-51392202181S.pdf UR - http://cherry.chem.bg.ac.rs/handle/123456789/5154 AB - In this paper, the previously synthesized Cu(II) complex ([CuL1(N3) (CH3OH)]BF4) with N,N,N-trimethyl-2-oxo-2-(2-(1-(thiazol-2-yl)ethylidene)hydrazinyl)ethan-1-aminium chloride, has been characterized and its biological activity has been studied in detail. The Cu(II) complex consists of ligand coordinated in a deprotonated, formally neutral zwitter-ionic form, via NNO atoms, one azido ligand and one methanol molecule. The Cu(II) complex was selected due to results of the cytotoxic activity, the brine shrimp test and DPPH radical scavenging activity, which were previously performed. The effects of Cu(II) complex on cell cycle phase distribution of cervical adenocarcinoma HeLa cells were investigated in order to examine the mechanisms of its anticancer activity. The measurement of intracellular ROS levels in HeLa and HaCaT cell lines were evaluated in order to explore their possible generation and the role in cytotoxic activity. The possible anti-invasive and anti-angiogenic properties of Cu(II) complex were evaluated. DNA binding experiments, including fluorescence displacement study and DNA cleavage experiments, were performed in order to obtain information on the type of DNA-metal complex interactions. © 2022 Serbian Chemical Society. All rights reserved. PB - Beograd : Srpsko hemijsko društvo T2 - Journal of the Serbian Chemical Society T1 - Evaluation of antitumor potential of Cu(II) complex with hydrazone of 2-acetylthiazole and Girard’s T reagent VL - 87 IS - 2 SP - 181 EP - 192 DO - 10.2298/JSC211203114S ER -
@article{ author = "Stevanović, Nevena and Jevtović, Mima and Mitić, Dragana and Matić, Ivana Z. and Crnogorac, Marija Đ. and Vujčić, Miroslava and Sladić, Dušan and Čobeljić, Božidar and Anđelković, Katarina K.", year = "2022", abstract = "In this paper, the previously synthesized Cu(II) complex ([CuL1(N3) (CH3OH)]BF4) with N,N,N-trimethyl-2-oxo-2-(2-(1-(thiazol-2-yl)ethylidene)hydrazinyl)ethan-1-aminium chloride, has been characterized and its biological activity has been studied in detail. The Cu(II) complex consists of ligand coordinated in a deprotonated, formally neutral zwitter-ionic form, via NNO atoms, one azido ligand and one methanol molecule. The Cu(II) complex was selected due to results of the cytotoxic activity, the brine shrimp test and DPPH radical scavenging activity, which were previously performed. The effects of Cu(II) complex on cell cycle phase distribution of cervical adenocarcinoma HeLa cells were investigated in order to examine the mechanisms of its anticancer activity. The measurement of intracellular ROS levels in HeLa and HaCaT cell lines were evaluated in order to explore their possible generation and the role in cytotoxic activity. The possible anti-invasive and anti-angiogenic properties of Cu(II) complex were evaluated. DNA binding experiments, including fluorescence displacement study and DNA cleavage experiments, were performed in order to obtain information on the type of DNA-metal complex interactions. © 2022 Serbian Chemical Society. All rights reserved.", publisher = "Beograd : Srpsko hemijsko društvo", journal = "Journal of the Serbian Chemical Society", title = "Evaluation of antitumor potential of Cu(II) complex with hydrazone of 2-acetylthiazole and Girard’s T reagent", volume = "87", number = "2", pages = "181-192", doi = "10.2298/JSC211203114S" }
Stevanović, N., Jevtović, M., Mitić, D., Matić, I. Z., Crnogorac, M. Đ., Vujčić, M., Sladić, D., Čobeljić, B.,& Anđelković, K. K.. (2022). Evaluation of antitumor potential of Cu(II) complex with hydrazone of 2-acetylthiazole and Girard’s T reagent. in Journal of the Serbian Chemical Society Beograd : Srpsko hemijsko društvo., 87(2), 181-192. https://doi.org/10.2298/JSC211203114S
Stevanović N, Jevtović M, Mitić D, Matić IZ, Crnogorac MĐ, Vujčić M, Sladić D, Čobeljić B, Anđelković KK. Evaluation of antitumor potential of Cu(II) complex with hydrazone of 2-acetylthiazole and Girard’s T reagent. in Journal of the Serbian Chemical Society. 2022;87(2):181-192. doi:10.2298/JSC211203114S .
Stevanović, Nevena, Jevtović, Mima, Mitić, Dragana, Matić, Ivana Z., Crnogorac, Marija Đ., Vujčić, Miroslava, Sladić, Dušan, Čobeljić, Božidar, Anđelković, Katarina K., "Evaluation of antitumor potential of Cu(II) complex with hydrazone of 2-acetylthiazole and Girard’s T reagent" in Journal of the Serbian Chemical Society, 87, no. 2 (2022):181-192, https://doi.org/10.2298/JSC211203114S . .