Electrochemical Determination of Morphine in Urine Samples by Tailoring FeWO4/CPE Sensor
Аутори
Ognjanović, MilošNikolić, Katarina
Bošković, Marko
Pastor, Ferenc
Popov, Nina
Marciuš, Marijan
Krehula, Stjepko
Antić, Bratislav
Stanković, Dalibor
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Morphine (MORPH) is natural alkaloid and mainly used as a pain reliever. Its monitoring in human body fluids is crucial for modern medicine. In this paper, we have developed an electrochemical sensor for submicromolar detection of MORPH. The sensor is based on modified carbon paste electrode (CPE) by investigating the FexW1-xO4 ratio in iron tungstate (FeWO4), as well as the ratio of this material in CPE. For the first time, the effect of the iron–tungsten ratio in terms of achieving the best possible electrochemical characteristics for the detection of an important molecule for humans was examined. Morphological and electrochemical characteristics of materials were studied. The best results were obtained using Fe1W3 and 7.5% of modifier in CPE. For MORPH detection, square wave voltammetry (SWV) was optimized. Under the optimized conditions, Fe1W3@CPE resulted in limit of detection (LOD) of the method of 0.58 µM and limit of quantification (LOQ) of 1.94 µM. The linear operating range b...etween 5 and 85 µM of MORPH in the Britton–Robinson buffer solution (BRBS) at pH 8 as supporting electrolyte was obtained. The Fe1W3@CPE sensor resulted in good selectivity and excellent repeatability with relative standard deviation (RSD) and was applied in real-world samples of human urine. Application for direct MORPH detection, without tedious sample pretreatment procedures, suggests that developed electrochemical sensor has appeared to be a suitable competitor for efficient, precise, and accurate monitoring of the MORPH in biological fluids.
Кључне речи:
iron tungstate / carbon paste electrode / electroanalysis / square wave voltammetry / morphine / real-world sampleИзвор:
Biosensors, 2022, 12, 11, 932-Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- EUREKA project E!13303
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Ognjanović, Miloš AU - Nikolić, Katarina AU - Bošković, Marko AU - Pastor, Ferenc AU - Popov, Nina AU - Marciuš, Marijan AU - Krehula, Stjepko AU - Antić, Bratislav AU - Stanković, Dalibor PY - 2022 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5682 AB - Morphine (MORPH) is natural alkaloid and mainly used as a pain reliever. Its monitoring in human body fluids is crucial for modern medicine. In this paper, we have developed an electrochemical sensor for submicromolar detection of MORPH. The sensor is based on modified carbon paste electrode (CPE) by investigating the FexW1-xO4 ratio in iron tungstate (FeWO4), as well as the ratio of this material in CPE. For the first time, the effect of the iron–tungsten ratio in terms of achieving the best possible electrochemical characteristics for the detection of an important molecule for humans was examined. Morphological and electrochemical characteristics of materials were studied. The best results were obtained using Fe1W3 and 7.5% of modifier in CPE. For MORPH detection, square wave voltammetry (SWV) was optimized. Under the optimized conditions, Fe1W3@CPE resulted in limit of detection (LOD) of the method of 0.58 µM and limit of quantification (LOQ) of 1.94 µM. The linear operating range between 5 and 85 µM of MORPH in the Britton–Robinson buffer solution (BRBS) at pH 8 as supporting electrolyte was obtained. The Fe1W3@CPE sensor resulted in good selectivity and excellent repeatability with relative standard deviation (RSD) and was applied in real-world samples of human urine. Application for direct MORPH detection, without tedious sample pretreatment procedures, suggests that developed electrochemical sensor has appeared to be a suitable competitor for efficient, precise, and accurate monitoring of the MORPH in biological fluids. PB - MDPI T2 - Biosensors T1 - Electrochemical Determination of Morphine in Urine Samples by Tailoring FeWO4/CPE Sensor VL - 12 IS - 11 SP - 932 DO - 10.3390/bios12110932 ER -
@article{ author = "Ognjanović, Miloš and Nikolić, Katarina and Bošković, Marko and Pastor, Ferenc and Popov, Nina and Marciuš, Marijan and Krehula, Stjepko and Antić, Bratislav and Stanković, Dalibor", year = "2022", abstract = "Morphine (MORPH) is natural alkaloid and mainly used as a pain reliever. Its monitoring in human body fluids is crucial for modern medicine. In this paper, we have developed an electrochemical sensor for submicromolar detection of MORPH. The sensor is based on modified carbon paste electrode (CPE) by investigating the FexW1-xO4 ratio in iron tungstate (FeWO4), as well as the ratio of this material in CPE. For the first time, the effect of the iron–tungsten ratio in terms of achieving the best possible electrochemical characteristics for the detection of an important molecule for humans was examined. Morphological and electrochemical characteristics of materials were studied. The best results were obtained using Fe1W3 and 7.5% of modifier in CPE. For MORPH detection, square wave voltammetry (SWV) was optimized. Under the optimized conditions, Fe1W3@CPE resulted in limit of detection (LOD) of the method of 0.58 µM and limit of quantification (LOQ) of 1.94 µM. The linear operating range between 5 and 85 µM of MORPH in the Britton–Robinson buffer solution (BRBS) at pH 8 as supporting electrolyte was obtained. The Fe1W3@CPE sensor resulted in good selectivity and excellent repeatability with relative standard deviation (RSD) and was applied in real-world samples of human urine. Application for direct MORPH detection, without tedious sample pretreatment procedures, suggests that developed electrochemical sensor has appeared to be a suitable competitor for efficient, precise, and accurate monitoring of the MORPH in biological fluids.", publisher = "MDPI", journal = "Biosensors", title = "Electrochemical Determination of Morphine in Urine Samples by Tailoring FeWO4/CPE Sensor", volume = "12", number = "11", pages = "932", doi = "10.3390/bios12110932" }
Ognjanović, M., Nikolić, K., Bošković, M., Pastor, F., Popov, N., Marciuš, M., Krehula, S., Antić, B.,& Stanković, D.. (2022). Electrochemical Determination of Morphine in Urine Samples by Tailoring FeWO4/CPE Sensor. in Biosensors MDPI., 12(11), 932. https://doi.org/10.3390/bios12110932
Ognjanović M, Nikolić K, Bošković M, Pastor F, Popov N, Marciuš M, Krehula S, Antić B, Stanković D. Electrochemical Determination of Morphine in Urine Samples by Tailoring FeWO4/CPE Sensor. in Biosensors. 2022;12(11):932. doi:10.3390/bios12110932 .
Ognjanović, Miloš, Nikolić, Katarina, Bošković, Marko, Pastor, Ferenc, Popov, Nina, Marciuš, Marijan, Krehula, Stjepko, Antić, Bratislav, Stanković, Dalibor, "Electrochemical Determination of Morphine in Urine Samples by Tailoring FeWO4/CPE Sensor" in Biosensors, 12, no. 11 (2022):932, https://doi.org/10.3390/bios12110932 . .