Post-translational modifications (PTMs) of major peanut allergen epitopes occurring as a result of purification process
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Peanut allergy affects a large portion of world population causing reactions ranging from
mild to severe. Seed storage proteins Ara h 1, Ara h 2, Ara h 3 and Ara h 6 are peanut
allergens, with well characterized IgE epitopes but little is known about PTMs effect on their
properties. Our aim was to investigate PTMs present on known epitopes of said purified
proteins using bottom-up proteomcs methods.
Out of the 4 allergens, Ara h 2 has the highest number of PTMs (14), while the most common
are hydroxylation Pro, dehydration and deamidation (N, Q). Peanut allergen epitopes are
indeed carriers of PTMs that could affect protein allergenicity and digestibility. Further
investigation on peanut extracts prepared in different ways is necessary in order to fully
understand the impact protein modifications could have on their allergenic potential.
Кључне речи:
major peanut allergens / mass spectrometry based proteomics / IgE epitopes / peanut allergy / PTMsИзвор:
55. savetovanje Srpskog hemijskog društva, Kratki izvodi radova, 2018, 66-66Издавач:
- Beograd : Srpsko hemijsko društvo
Финансирање / пројекти:
- Молекуларне особине и модификације неких респираторних и нутритивних алергена (RS-MESTD-Basic Research (BR or ON)-172024)
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - CONF AU - Mihailović, Jelena AU - Apostolović, Danijela AU - Smiljanić, Katarina AU - Ćirković-Veličković, Tanja PY - 2018 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5713 AB - Peanut allergy affects a large portion of world population causing reactions ranging from mild to severe. Seed storage proteins Ara h 1, Ara h 2, Ara h 3 and Ara h 6 are peanut allergens, with well characterized IgE epitopes but little is known about PTMs effect on their properties. Our aim was to investigate PTMs present on known epitopes of said purified proteins using bottom-up proteomcs methods. Out of the 4 allergens, Ara h 2 has the highest number of PTMs (14), while the most common are hydroxylation Pro, dehydration and deamidation (N, Q). Peanut allergen epitopes are indeed carriers of PTMs that could affect protein allergenicity and digestibility. Further investigation on peanut extracts prepared in different ways is necessary in order to fully understand the impact protein modifications could have on their allergenic potential. PB - Beograd : Srpsko hemijsko društvo C3 - 55. savetovanje Srpskog hemijskog društva, Kratki izvodi radova T1 - Post-translational modifications (PTMs) of major peanut allergen epitopes occurring as a result of purification process SP - 66 EP - 66 UR - https://hdl.handle.net/21.15107/rcub_cherry_5713 ER -
@conference{ author = "Mihailović, Jelena and Apostolović, Danijela and Smiljanić, Katarina and Ćirković-Veličković, Tanja", year = "2018", abstract = "Peanut allergy affects a large portion of world population causing reactions ranging from mild to severe. Seed storage proteins Ara h 1, Ara h 2, Ara h 3 and Ara h 6 are peanut allergens, with well characterized IgE epitopes but little is known about PTMs effect on their properties. Our aim was to investigate PTMs present on known epitopes of said purified proteins using bottom-up proteomcs methods. Out of the 4 allergens, Ara h 2 has the highest number of PTMs (14), while the most common are hydroxylation Pro, dehydration and deamidation (N, Q). Peanut allergen epitopes are indeed carriers of PTMs that could affect protein allergenicity and digestibility. Further investigation on peanut extracts prepared in different ways is necessary in order to fully understand the impact protein modifications could have on their allergenic potential.", publisher = "Beograd : Srpsko hemijsko društvo", journal = "55. savetovanje Srpskog hemijskog društva, Kratki izvodi radova", title = "Post-translational modifications (PTMs) of major peanut allergen epitopes occurring as a result of purification process", pages = "66-66", url = "https://hdl.handle.net/21.15107/rcub_cherry_5713" }
Mihailović, J., Apostolović, D., Smiljanić, K.,& Ćirković-Veličković, T.. (2018). Post-translational modifications (PTMs) of major peanut allergen epitopes occurring as a result of purification process. in 55. savetovanje Srpskog hemijskog društva, Kratki izvodi radova Beograd : Srpsko hemijsko društvo., 66-66. https://hdl.handle.net/21.15107/rcub_cherry_5713
Mihailović J, Apostolović D, Smiljanić K, Ćirković-Veličković T. Post-translational modifications (PTMs) of major peanut allergen epitopes occurring as a result of purification process. in 55. savetovanje Srpskog hemijskog društva, Kratki izvodi radova. 2018;:66-66. https://hdl.handle.net/21.15107/rcub_cherry_5713 .
Mihailović, Jelena, Apostolović, Danijela, Smiljanić, Katarina, Ćirković-Veličković, Tanja, "Post-translational modifications (PTMs) of major peanut allergen epitopes occurring as a result of purification process" in 55. savetovanje Srpskog hemijskog društva, Kratki izvodi radova (2018):66-66, https://hdl.handle.net/21.15107/rcub_cherry_5713 .