Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts
Аутори
Topakas, EvangelosSiaperas, Romanos
Taxeidis, George
Makryniotis, Konstantinos
Guzik, Maciej
Maslak, Veselin
Beškoski, Vladimir
Lončarević, Branka
Ilić-Tomić, Tatjana
Milivojević, Dušan
Škaro Bogojević, Sanja
Pantelić, Brana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually ...no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options.
Кључне речи:
bioremediation / biodegradation / Amycolatopsis mediterranei / ecotoxicology / model substrate / biocatalysis / polyurethaneИзвор:
Catalysts, 2023, 13, 2, 278-Издавач:
- MDPI
Финансирање / пројекти:
- The European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No. 870292 (BioICEP)
- The National Natural Science Foundation of China (Nos. 31961133016)
- The National Natural Science Foundation of China (Nos. 31961133015)
- The National Natural Science Foundation of China (Nos. 31961133014)
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Topakas, Evangelos AU - Siaperas, Romanos AU - Taxeidis, George AU - Makryniotis, Konstantinos AU - Guzik, Maciej AU - Maslak, Veselin AU - Beškoski, Vladimir AU - Lončarević, Branka AU - Ilić-Tomić, Tatjana AU - Milivojević, Dušan AU - Škaro Bogojević, Sanja AU - Pantelić, Brana PY - 2023 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5969 AB - Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options. PB - MDPI T2 - Catalysts T1 - Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts VL - 13 IS - 2 SP - 278 DO - 10.3390/catal13020278 ER -
@article{ author = "Topakas, Evangelos and Siaperas, Romanos and Taxeidis, George and Makryniotis, Konstantinos and Guzik, Maciej and Maslak, Veselin and Beškoski, Vladimir and Lončarević, Branka and Ilić-Tomić, Tatjana and Milivojević, Dušan and Škaro Bogojević, Sanja and Pantelić, Brana", year = "2023", abstract = "Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options.", publisher = "MDPI", journal = "Catalysts", title = "Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts", volume = "13", number = "2", pages = "278", doi = "10.3390/catal13020278" }
Topakas, E., Siaperas, R., Taxeidis, G., Makryniotis, K., Guzik, M., Maslak, V., Beškoski, V., Lončarević, B., Ilić-Tomić, T., Milivojević, D., Škaro Bogojević, S.,& Pantelić, B.. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. in Catalysts MDPI., 13(2), 278. https://doi.org/10.3390/catal13020278
Topakas E, Siaperas R, Taxeidis G, Makryniotis K, Guzik M, Maslak V, Beškoski V, Lončarević B, Ilić-Tomić T, Milivojević D, Škaro Bogojević S, Pantelić B. Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. in Catalysts. 2023;13(2):278. doi:10.3390/catal13020278 .
Topakas, Evangelos, Siaperas, Romanos, Taxeidis, George, Makryniotis, Konstantinos, Guzik, Maciej, Maslak, Veselin, Beškoski, Vladimir, Lončarević, Branka, Ilić-Tomić, Tatjana, Milivojević, Dušan, Škaro Bogojević, Sanja, Pantelić, Brana, "Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts" in Catalysts, 13, no. 2 (2023):278, https://doi.org/10.3390/catal13020278 . .