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Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation
dc.creator | Goff, Miriam | |
dc.creator | Nikodinović-Runić, Jasmina | |
dc.creator | O'Connor, Kevin E. | |
dc.date.accessioned | 2018-11-22T00:14:10Z | |
dc.date.available | 2018-11-22T00:14:10Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 0378-1097 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/607 | |
dc.description.abstract | A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were characterized phenotypically. CA-3-126, a mutant disrupted in a stress-related protein Clp protease subunit ClpA, demonstrated greater decreases in PHA accumulation compared with the wild type at reduced and elevated temperatures under PHA-accumulating growth conditions. CA-3-M, which is affected in the aminotransferase class I enzyme, accumulated reduced levels of PHA relative to the wild type and had lower growth yields on all carbon sources tested. Mutant CA-3-M produced up to 10-fold higher levels of lipopolysaccharide relative to the wild type and exhibited 1.2-fold lower aminotransferase activity with phenylalanine as a substrate compared with the wild-type strain. The composition of the lipopolysaccharide produced by the mutant differed from that produced by the wild-type strain. Growth and PHA accumulation by CA-3-M was the same as the wild type when the nitrogen concentration in the medium was increased to 265 mg N L(-1). | en |
dc.publisher | Wiley-Blackwell Publishing, Inc, Malden | |
dc.relation | Higher Education Authority, grant (PRTLI4) in the Centre for Synthesis and Chemical Biology (CSCB) at University College, Dublin. | |
dc.relation | J.N.-R. is funded by a research grant from the Environmental Protection Agency Ireland. | |
dc.relation | Carbohydrate Research Center is supported in part by the Department of Energy-funded (DE-FG09-93ER-20097) Center for Plant and Microbial Complex Carbohydrates. | |
dc.rights | restrictedAccess | |
dc.source | FEMS Microbiology Letters / Federation of European Microbiological Societies | |
dc.subject | polyhydroxyalkanoate | en |
dc.subject | lipopolysaccharide | en |
dc.subject | Pseudomonas putida CA-3 | en |
dc.title | Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Гофф, Мириам; Никодиновић-Рунић, Јасмина; О'Цоннор, Кевин Е.; | |
dc.citation.volume | 292 | |
dc.citation.issue | 2 | |
dc.citation.spage | 297 | |
dc.citation.epage | 305 | |
dc.identifier.wos | 000263452300020 | |
dc.identifier.doi | 10.1111/j.1574-6968.2009.01504.x | |
dc.citation.other | 292(2): 297-305 | |
dc.citation.rank | M23 | |
dc.identifier.pmid | 19187205 | |
dc.description.other | Free full text: [https://doi.org/10.1111/j.1574-6968.2009.01504.x] | |
dc.type.version | publishedVersion | en |
dc.identifier.scopus | 2-s2.0-60349127051 |