Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines
Аутори
Gospodinova, Z.Antov, G.
Novaković, M.
Tešević, Vele
Krasteva, N.
Pavlov, D.
Valcheva-Kuzmanova, S.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
AbstractBackground and objectiveCotinus coggygria Scop. is a valuable medicinal plant species with pronounced pharmacological potential due to its numerous biological activities. The herb is characterized by a high content of polyphenols among which is fustin. The anticancer activities of fustin, however, are extremely weakly studied. The aim of the present study was to investigate the in vitro antiproliferative potential of fustin isolated from the heartwood of C. coggygria against cell lines originating from two of the most common cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26).Materials and methodsCell growth inhibitory properties of fustin were examined by MTT assay. Subsequently, phase-contrast and fluorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin cell line.ResultsThe obtained results showed that fustin reduced the proliferation of all studied cell lines. The highes...t cytostatic effect was registered towards breast cancer MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was least affected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer MDA-MB-231 cells indicated decreased density of cell
Извор:
Acta Medica Bulgarica, 2023, 50, 1, 27-33Издавач:
- Sciendo
Финансирање / пројекти:
- Bulgarian National Science Fund, Ministry of Education and Science
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Gospodinova, Z. AU - Antov, G. AU - Novaković, M. AU - Tešević, Vele AU - Krasteva, N. AU - Pavlov, D. AU - Valcheva-Kuzmanova, S. PY - 2023 UR - http://cherry.chem.bg.ac.rs/handle/123456789/6218 AB - AbstractBackground and objectiveCotinus coggygria Scop. is a valuable medicinal plant species with pronounced pharmacological potential due to its numerous biological activities. The herb is characterized by a high content of polyphenols among which is fustin. The anticancer activities of fustin, however, are extremely weakly studied. The aim of the present study was to investigate the in vitro antiproliferative potential of fustin isolated from the heartwood of C. coggygria against cell lines originating from two of the most common cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26).Materials and methodsCell growth inhibitory properties of fustin were examined by MTT assay. Subsequently, phase-contrast and fluorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin cell line.ResultsThe obtained results showed that fustin reduced the proliferation of all studied cell lines. The highest cytostatic effect was registered towards breast cancer MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was least affected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer MDA-MB-231 cells indicated decreased density of cell PB - Sciendo T2 - Acta Medica Bulgarica T1 - Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines VL - 50 IS - 1 SP - 27 EP - 33 DO - 10.2478/amb-2023-0004 ER -
@article{ author = "Gospodinova, Z. and Antov, G. and Novaković, M. and Tešević, Vele and Krasteva, N. and Pavlov, D. and Valcheva-Kuzmanova, S.", year = "2023", abstract = "AbstractBackground and objectiveCotinus coggygria Scop. is a valuable medicinal plant species with pronounced pharmacological potential due to its numerous biological activities. The herb is characterized by a high content of polyphenols among which is fustin. The anticancer activities of fustin, however, are extremely weakly studied. The aim of the present study was to investigate the in vitro antiproliferative potential of fustin isolated from the heartwood of C. coggygria against cell lines originating from two of the most common cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26).Materials and methodsCell growth inhibitory properties of fustin were examined by MTT assay. Subsequently, phase-contrast and fluorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin cell line.ResultsThe obtained results showed that fustin reduced the proliferation of all studied cell lines. The highest cytostatic effect was registered towards breast cancer MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was least affected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer MDA-MB-231 cells indicated decreased density of cell", publisher = "Sciendo", journal = "Acta Medica Bulgarica", title = "Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines", volume = "50", number = "1", pages = "27-33", doi = "10.2478/amb-2023-0004" }
Gospodinova, Z., Antov, G., Novaković, M., Tešević, V., Krasteva, N., Pavlov, D.,& Valcheva-Kuzmanova, S.. (2023). Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines. in Acta Medica Bulgarica Sciendo., 50(1), 27-33. https://doi.org/10.2478/amb-2023-0004
Gospodinova Z, Antov G, Novaković M, Tešević V, Krasteva N, Pavlov D, Valcheva-Kuzmanova S. Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines. in Acta Medica Bulgarica. 2023;50(1):27-33. doi:10.2478/amb-2023-0004 .
Gospodinova, Z., Antov, G., Novaković, M., Tešević, Vele, Krasteva, N., Pavlov, D., Valcheva-Kuzmanova, S., "Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines" in Acta Medica Bulgarica, 50, no. 1 (2023):27-33, https://doi.org/10.2478/amb-2023-0004 . .