Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome
Аутори
Apostolović, DanijelaGrundström, Jeanette
Kiewiet, Mensiena B. Gea
Peruško, Marija
Hamsten, Carl
Starkhammar, Maria
Paulie, Staffan
Hage, Marianne van
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Tick bites have been shown to transmit a novel form of severe food allergy, the galactose-α-1,3-galactose (α-Gal) syndrome (AGS). Cellular responses to α-Gal in patients with AGS have, to date, not been thoroughly scrutinized. Therefore, we investigated T and B cell proliferation, activation, and cytokine profiles in response to tick protein extract (TE) and α-Gal-free TE in patients with AGS and in healthy controls. T and B cells from both patients and controls proliferated in response to TE, but significantly more in patients with AGS. B cell proliferation, but not T cell proliferation, in patients with AGS was reduced by removing α-Gal from the TE. In addition, TE induced a clear Th2 cytokine profile in patients with AGS. Expression of CD23 by B cells correlated only to T cell proliferation. However, both B cell proliferation and CD23 expression were reduced when CD40L and IL-4 were blocked. A large portion of the IgG1 and IgE antibodies binding TE in patients with AGS were directed... against the α-Gal epitope. We have, for what we believe to be the first time, investigated T and B cell responses to α-Gal carrying tick proteins in patients with AGS, which will be essential for the understanding of the immune response against an allergenic carbohydrate transmitted by ticks.
Кључне речи:
tick / T cells / B cell / α-GalИзвор:
The Journal of Clinical Investigation, 2023, 133, 6Издавач:
- American Society for Clinical Investigation
Финансирање / пројекти:
- Swedish Research Council grants 2019-01060 (MVH)
- Swedish Asthma and Allergy Association’s Research Foundation
- Swedish Asthma and Allergy Association’s Research Foundation
- Swedish Heart-Lung Foundation (MVH); The Hesselman Foundation
- EU H2020 project FoodEnTwin grant GA810752
- The Konsul Th C Bergh Foundation (DA)
- ore Nilsons Foundation for Medical Research
- The Swedish Cancer and Allergy Foundation
- The Magnus Bergvall Foundation
- Eva and Oscar Ahrén’s Foundation
- Karolinska Institutet Research Foundation
- The Swedish Medical Society
Колекције
Институција/група
Inovacioni centar / Innovation CentreTY - JOUR AU - Apostolović, Danijela AU - Grundström, Jeanette AU - Kiewiet, Mensiena B. Gea AU - Peruško, Marija AU - Hamsten, Carl AU - Starkhammar, Maria AU - Paulie, Staffan AU - Hage, Marianne van PY - 2023 UR - http://cherry.chem.bg.ac.rs/handle/123456789/6244 AB - Tick bites have been shown to transmit a novel form of severe food allergy, the galactose-α-1,3-galactose (α-Gal) syndrome (AGS). Cellular responses to α-Gal in patients with AGS have, to date, not been thoroughly scrutinized. Therefore, we investigated T and B cell proliferation, activation, and cytokine profiles in response to tick protein extract (TE) and α-Gal-free TE in patients with AGS and in healthy controls. T and B cells from both patients and controls proliferated in response to TE, but significantly more in patients with AGS. B cell proliferation, but not T cell proliferation, in patients with AGS was reduced by removing α-Gal from the TE. In addition, TE induced a clear Th2 cytokine profile in patients with AGS. Expression of CD23 by B cells correlated only to T cell proliferation. However, both B cell proliferation and CD23 expression were reduced when CD40L and IL-4 were blocked. A large portion of the IgG1 and IgE antibodies binding TE in patients with AGS were directed against the α-Gal epitope. We have, for what we believe to be the first time, investigated T and B cell responses to α-Gal carrying tick proteins in patients with AGS, which will be essential for the understanding of the immune response against an allergenic carbohydrate transmitted by ticks. PB - American Society for Clinical Investigation T2 - The Journal of Clinical Investigation T1 - Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome VL - 133 IS - 6 DO - 10.1172/JCI158357 ER -
@article{ author = "Apostolović, Danijela and Grundström, Jeanette and Kiewiet, Mensiena B. Gea and Peruško, Marija and Hamsten, Carl and Starkhammar, Maria and Paulie, Staffan and Hage, Marianne van", year = "2023", abstract = "Tick bites have been shown to transmit a novel form of severe food allergy, the galactose-α-1,3-galactose (α-Gal) syndrome (AGS). Cellular responses to α-Gal in patients with AGS have, to date, not been thoroughly scrutinized. Therefore, we investigated T and B cell proliferation, activation, and cytokine profiles in response to tick protein extract (TE) and α-Gal-free TE in patients with AGS and in healthy controls. T and B cells from both patients and controls proliferated in response to TE, but significantly more in patients with AGS. B cell proliferation, but not T cell proliferation, in patients with AGS was reduced by removing α-Gal from the TE. In addition, TE induced a clear Th2 cytokine profile in patients with AGS. Expression of CD23 by B cells correlated only to T cell proliferation. However, both B cell proliferation and CD23 expression were reduced when CD40L and IL-4 were blocked. A large portion of the IgG1 and IgE antibodies binding TE in patients with AGS were directed against the α-Gal epitope. We have, for what we believe to be the first time, investigated T and B cell responses to α-Gal carrying tick proteins in patients with AGS, which will be essential for the understanding of the immune response against an allergenic carbohydrate transmitted by ticks.", publisher = "American Society for Clinical Investigation", journal = "The Journal of Clinical Investigation", title = "Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome", volume = "133", number = "6", doi = "10.1172/JCI158357" }
Apostolović, D., Grundström, J., Kiewiet, M. B. G., Peruško, M., Hamsten, C., Starkhammar, M., Paulie, S.,& Hage, M. v.. (2023). Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome. in The Journal of Clinical Investigation American Society for Clinical Investigation., 133(6). https://doi.org/10.1172/JCI158357
Apostolović D, Grundström J, Kiewiet MBG, Peruško M, Hamsten C, Starkhammar M, Paulie S, Hage MV. Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome. in The Journal of Clinical Investigation. 2023;133(6). doi:10.1172/JCI158357 .
Apostolović, Danijela, Grundström, Jeanette, Kiewiet, Mensiena B. Gea, Peruško, Marija, Hamsten, Carl, Starkhammar, Maria, Paulie, Staffan, Hage, Marianne van, "Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome" in The Journal of Clinical Investigation, 133, no. 6 (2023), https://doi.org/10.1172/JCI158357 . .