Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment
Само за регистроване кориснике
2023
Аутори
Matić, DraganaVlahović, Milena
Grčić, Anja
Filipović, Aleksandra
Ilijin, Larisa
Mrdaković, Marija
Mutić, Jelena
Đurđić, Slađana Z.
Perić-Mataruga, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Long-term exposure of populations to pollution may result in enhanced ability to cope with environmental stress. To compare the responses of two Lymantria dispar populations living in unpolluted and polluted forests (UP and PP, respectively), we chronically exposed larvae to cadmium at concentrations of 50 and 100 μg Cd/g dry food (Cd1 and Cd2, respectively). We examined cadmium accumulation in the midgut and hemolymph, activities of superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatases (ALP) in the midgut, as well as Hsp70 protein expression in the midgut, hemolymph, and brain and evaluated these parameters as biomarkers of cadmium contamination. Larvae from PP, fed a control diet, showed higher activity of SOD and increased Hsp70 expression compared with larvae from UP. Excessive amounts of Cd were accumulated in the midgut of all Cd-fed larvae, whereas Cd content in the hemolymph was elevated only in larvae from PP after Cd2 treatment. In larvae from UP, Cd2 treatmen...t decreased the activity of CAT and induced the expression of Hsp70 in the midgut and hemolymph. In larvae from PP, exposure to both Cd concentrations strongly attenuated SOD and CAT activities, while Hsp70 expression was not induced in any organ/tissue. Cd did not affect ALP activity in either population. Midgut Cd content proved to be a suitable indicator of Cd contamination for both polluted and unpolluted habitats.
Кључне речи:
Antioxidative enzymes / Biomarker / Cadmium / Cadmium accumulation / Hsp70Извор:
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2023, 273, 109721-Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Matić, Dragana AU - Vlahović, Milena AU - Grčić, Anja AU - Filipović, Aleksandra AU - Ilijin, Larisa AU - Mrdaković, Marija AU - Mutić, Jelena AU - Đurđić, Slađana Z. AU - Perić-Mataruga, Vesna PY - 2023 UR - http://cherry.chem.bg.ac.rs/handle/123456789/6329 AB - Long-term exposure of populations to pollution may result in enhanced ability to cope with environmental stress. To compare the responses of two Lymantria dispar populations living in unpolluted and polluted forests (UP and PP, respectively), we chronically exposed larvae to cadmium at concentrations of 50 and 100 μg Cd/g dry food (Cd1 and Cd2, respectively). We examined cadmium accumulation in the midgut and hemolymph, activities of superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatases (ALP) in the midgut, as well as Hsp70 protein expression in the midgut, hemolymph, and brain and evaluated these parameters as biomarkers of cadmium contamination. Larvae from PP, fed a control diet, showed higher activity of SOD and increased Hsp70 expression compared with larvae from UP. Excessive amounts of Cd were accumulated in the midgut of all Cd-fed larvae, whereas Cd content in the hemolymph was elevated only in larvae from PP after Cd2 treatment. In larvae from UP, Cd2 treatment decreased the activity of CAT and induced the expression of Hsp70 in the midgut and hemolymph. In larvae from PP, exposure to both Cd concentrations strongly attenuated SOD and CAT activities, while Hsp70 expression was not induced in any organ/tissue. Cd did not affect ALP activity in either population. Midgut Cd content proved to be a suitable indicator of Cd contamination for both polluted and unpolluted habitats. PB - Elsevier T2 - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology T1 - Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment VL - 273 SP - 109721 DO - 10.1016/j.cbpc.2023.109721 ER -
@article{ author = "Matić, Dragana and Vlahović, Milena and Grčić, Anja and Filipović, Aleksandra and Ilijin, Larisa and Mrdaković, Marija and Mutić, Jelena and Đurđić, Slađana Z. and Perić-Mataruga, Vesna", year = "2023", abstract = "Long-term exposure of populations to pollution may result in enhanced ability to cope with environmental stress. To compare the responses of two Lymantria dispar populations living in unpolluted and polluted forests (UP and PP, respectively), we chronically exposed larvae to cadmium at concentrations of 50 and 100 μg Cd/g dry food (Cd1 and Cd2, respectively). We examined cadmium accumulation in the midgut and hemolymph, activities of superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatases (ALP) in the midgut, as well as Hsp70 protein expression in the midgut, hemolymph, and brain and evaluated these parameters as biomarkers of cadmium contamination. Larvae from PP, fed a control diet, showed higher activity of SOD and increased Hsp70 expression compared with larvae from UP. Excessive amounts of Cd were accumulated in the midgut of all Cd-fed larvae, whereas Cd content in the hemolymph was elevated only in larvae from PP after Cd2 treatment. In larvae from UP, Cd2 treatment decreased the activity of CAT and induced the expression of Hsp70 in the midgut and hemolymph. In larvae from PP, exposure to both Cd concentrations strongly attenuated SOD and CAT activities, while Hsp70 expression was not induced in any organ/tissue. Cd did not affect ALP activity in either population. Midgut Cd content proved to be a suitable indicator of Cd contamination for both polluted and unpolluted habitats.", publisher = "Elsevier", journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology", title = "Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment", volume = "273", pages = "109721", doi = "10.1016/j.cbpc.2023.109721" }
Matić, D., Vlahović, M., Grčić, A., Filipović, A., Ilijin, L., Mrdaković, M., Mutić, J., Đurđić, S. Z.,& Perić-Mataruga, V.. (2023). Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology Elsevier., 273, 109721. https://doi.org/10.1016/j.cbpc.2023.109721
Matić D, Vlahović M, Grčić A, Filipović A, Ilijin L, Mrdaković M, Mutić J, Đurđić SZ, Perić-Mataruga V. Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2023;273:109721. doi:10.1016/j.cbpc.2023.109721 .
Matić, Dragana, Vlahović, Milena, Grčić, Anja, Filipović, Aleksandra, Ilijin, Larisa, Mrdaković, Marija, Mutić, Jelena, Đurđić, Slađana Z., Perić-Mataruga, Vesna, "Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 273 (2023):109721, https://doi.org/10.1016/j.cbpc.2023.109721 . .